Rabbit MEK1, MEK2 Polyclonal Antibody | anti-Map2k2 antibody

MEK1, MEK2, phosphorylated (Ser217/221) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mitogen-activat

Cat. Num. AAA625933

• Gene Names: Map2k2; MK2; MEK2; Prkmk2; AA589381
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MEK1; MEK2; Polyclonal Antibody; phosphorylated (Ser217/221) (MAPK/ERK Kinase 1; MEK 1; Dual Specificity Mitogen-activated Protein Kinase Kinase 1; MAP Kinase Kinase 1; MAP2K1; MAPKK 1; MKK1; PRKMK1; ERK Activator Kinase 1 / MAPK/ERK Kinase 2; Dual Specificity Mitogen-activat; Anti -MEK1; anti-Map2k2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. Does not crossreact with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217, but not with MEK1/2 singly phosphorylated at Ser221. Species Crossreactivity: Human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-Map2k2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50
• Immunogen: Synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2 (KLH coupled). MW: 45kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum for the indicated times, using MBS625933(upper) or M2865-02(lower).)

Related Product Information for anti-Map2k2 antibody

MEK1 and MEK2, also called MAP or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 (in the activation loop of subdomain VIII), by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx. Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC12 cells. MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. Phospho-MEK1/2 (Ser217/221) selectively recognizes active MEK, i.e., only when phosphorylated at Ser217/221, and hence is an excellent marker of MEK1/2 activity.

Product Categories/Family for anti-Map2k2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 545203
• NCBI GeneID: 26396
• UniProt Accession #: Q63932; Q9D7B0
• Molecular Weight: 44,402 Da
• NCBI Official Full Name: MEK2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: Map2k2
• NCBI Official Synonym Symbols: MK2; MEK2; Prkmk2; AA589381
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2; MEK 2; MAPKK 2; MAPK/ERK kinase 2; MAP kinase kinase 2; MAP kinase/Erk kinase; ERK activator kinase 2; mitogen activated protein kinase kinase 2; protein kinase, mitogen activated, kinase 2, p45
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k2
• UniProt Synonym Gene Names: Mek2; Mkk2; Prkmk2; MAP kinase kinase 2; MAPKK 2; MEK 2
• UniProt Entry Name: MP2K2_MOUSE

Uniprot Description

MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; STE group; STE7 family

Cellular Component: peroxisomal membrane; Golgi apparatus; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; intercellular junction; cell cortex; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus

Molecular Function: nucleotide binding; protein kinase activity; PDZ domain binding; MAP kinase kinase activity; transferase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; kinase activity; protein complex scaffold; ATP binding

Biological Process: regulation of axon regeneration; regulation of stress-activated MAPK cascade; activation of MAPK activity; MAPKKK cascade; positive regulation of axonogenesis; protein amino acid phosphorylation; phosphorylation