Goat anti-Mouse Integrin alpha8 Polyclonal Antibody | anti-ITGA8 antibody
Integrin alpha8 (Biotin)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions. In this format, shows <5% crossreactivity with recombinant human Integrin alpha5 and recombinant mouse Integrin alpha5, and <1% crossreactivity with recombinant mouse Integrin alphaV.
Immunocytochemistry: 10ug/ml detects Integrin alpha8 in 4T1 mouse mammary carcinoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted I7661-36V1 for 3 hours at RT followed by a fluorochrome-conjugated streptavidin at RT for 1 hour. Between each step, cells were washed with PBS, 0.1% BSA.
NCBI and Uniprot Product Information
NCBI Description
Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]
Uniprot Description
ITGA8: Integrin alpha-8/beta-1 functions in the genesis of kidney and probably of other organs by regulating the recruitment of mesenchymal cells into epithelial structures. It recognizes the sequence R-G-D in a wide array of ligands including TNC, FN1, SPP1 TGFB1, TGFB3 and VTN. NPNT is probably its functional ligand in kidney genesis. Neuronal receptor for TNC it mediates cell-cell interactions and regulates neurite outgrowth of sensory and motor neurons. Belongs to the integrin alpha chain family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: focal adhesion; cell surface; endoplasmic reticulum; apical part of cell; postsynaptic density; plasma membrane; perikaryon; integrin complex
Molecular Function: metal ion binding
Biological Process: integrin-mediated signaling pathway; smooth muscle development; establishment of protein localization; cell-cell adhesion; extracellular matrix organization and biogenesis; inner ear morphogenesis; cell projection organization and biogenesis; cell-matrix adhesion; positive regulation of transforming growth factor beta receptor signaling pathway; mesodermal cell differentiation; brain development; kidney development; metanephros development; memory
Disease: Renal Hypodysplasia/aplasia 1
Research Articles on ITGA8
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Product Notes
The ITGA8 itga8 (Catalog #AAA614175) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Integrin alpha8 (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Dilution: Flow cytometry: 50ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume 200ul. Tested on 4T1 mouse mammary carcinoma cells. Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions. In this format, shows <5% crossreactivity with recombinant human Integrin alpha5 and recombinant mouse Integrin alpha5, and <1% crossreactivity with recombinant mouse Integrin alphaV. Immunocytochemistry: 10ug/ml detects Integrin alpha8 in 4T1 mouse mammary carcinoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted I7661-36V1 for 3 hours at RT followed by a fluorochrome-conjugated streptavidin at RT for 1 hour. Between each step, cells were washed with PBS, 0.1% BSA. Researchers should empirically determine the suitability of the ITGA8 itga8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin alpha8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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