SDS-Page
(Detection of FOLR1 using MBS620439. Cell lysates were resolved by SDS-PAGE, transferred to a membrane and immunoblotted with 1ug/ml MBS620439.)
Goat anti-Human Folate receptor 1 Polyclonal Antibody | anti-FOLR1 antibody
Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Folate receptor 1; Polyclonal Antibody; Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein; FBP; Folate receptor; adult; Folate receptor alpha; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18); Anti -Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein; anti-FOLR1 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FOLR1. Shows < 5% crossreactivity with recombinant human FOLR2 and FOLR3 in direct ELISAs.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul PBS.
Applicable Applications for anti-FOLR1 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, ELISA and Western Blot.
Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 MCR-7 cells in a total volume 200ul. Wash cells in Flow Cytometry Staining Buffer (F4568-10) or other BSA-supplemented saline buffer.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.
Western Blot: 1ug/ml
Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 MCR-7 cells in a total volume 200ul. Wash cells in Flow Cytometry Staining Buffer (F4568-10) or other BSA-supplemented saline buffer.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.
Western Blot: 1ug/ml
Immunogen
Recombinant corresponding to aa25-233 of human FOLR1 expressed in CHO cells (P15328).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of FOLR1 using MBS620439. Cell lysates were resolved by SDS-PAGE, transferred to a membrane and immunoblotted with 1ug/ml MBS620439.)
SDS-Page
(Detection of FOLR1 using MBS620439. Cell lysates were resolved by SDS-PAGE, transferred to a membrane and immunoblotted with 1ug/ml MBS620439.)
Testing Data
(MCF-7 cells were stained with anti-human FOLR1 (MBS620439, filled histogram), or control antibody (open histogram) followed by PE-conjugated anti-goat antibody.)
Testing Data
(MCF-7 cells were stained with anti-human FOLR1 (MBS620439, filled histogram), or control antibody (open histogram) followed by PE-conjugated anti-goat antibody.)
Related Product Information for anti-FOLR1 antibody
Folate receptor 1 (FOLR1; also folate receptor alpha, folate binding protein 1, and MOv18) is a 37-42kD member of the folate receptor family. FOLR1 is expressed in reticulocytes and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell. The human FOLR1 preproprecursor is 257aa in length. It codes for a GPI-linked glycoprotein that is 210 aa in size (aa25-234). There are two forms of FOLR1. One is GPI-linked, while the other is a soluble MMP cleavage product (aa25-226). There is one potential alternate splice form that shows a five aa substitution for aa 121-205. Over aa 25-233, human FOLR1 shares 83% aa identity with mouse FOLR1.
Product Categories/Family for anti-FOLR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
folate receptor alpha
NCBI Official Symbol
FOLR1
NCBI Protein Information
folate receptor alpha
Protein Family
Similar Products
Product Notes
The FOLR1 (Catalog #AAA620439) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Folate receptor 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, ELISA and Western Blot. Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 MCR-7 cells in a total volume 200ul. Wash cells in Flow Cytometry Staining Buffer (F4568-10) or other BSA-supplemented saline buffer. Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well. Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the FOLR1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Folate receptor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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