Sheep anti-Human Integrin, beta7 Polyclonal Antibody | anti-ITGB7 antibody

Integrin, beta7 (Integrin BetaP, M290 IEL Ag)

Cat. Num. AAA624208

• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Integrin; beta7; Polyclonal Antibody; beta7 (Integrin BetaP; M290 IEL Ag); Anti -Integrin; anti-ITGB7 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Integrin beta7. Shows ~20% cross-reactivity with recombinant mouse Integrin beta7 and <1% cross-reactivity with recombinant human Integrin beta2, beta5 and beta6.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

• Applicable Applications for anti-ITGB7 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry.; Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.2ng/well.; Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.; Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul. Tested using blood derived lymphocytes.
• Immunogen: CHO cell-derived recombinant human Integrin Beta7 long isoform, extracellular domain (aa20-723, Accession # P26010)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Blood-derived lymphocytes were stained with MBS624208 (filled histogram) or control antibody (open histogram) followed by NL557-conjugated anti-sheep IgG.)

Related Product Information for anti-ITGB7 antibody

Human Integrin Beta7 (also Integrin BetaP and M290 IEL Ag) is a 120kD member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa20-723) and a 52aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa150-389) and four cysteine-rich EGF-like repeats. One splice variant exists that shows a deletion of aa501-648. Integrin Beta7 forms a nondisulfide linked heterodimer with alpha4 and alphaE integrins. Alpha4Beta7 binds fibronectin, VCAM-1 and MADCAM1, while alphaEBeta7 binds E-cadherin. The ECD of human Beta7 is 87% aa identical to the ECD of mouse Beta7.

Product Categories/Family for anti-ITGB7 antibody

Antibodies; Abs to Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 119617081
• NCBI GeneID: 3695
• UniProt Accession #: P26010; Q9UCP7; Q9UCS7
• Molecular Weight: 86,903 Da
• NCBI Official Full Name: integrin, beta 7
• NCBI Official Synonym Full Names: integrin, beta 7
• NCBI Official Symbol: ITGB7
• NCBI Protein Information: integrin beta-7; integrin beta 7 subunit; gut homing receptor beta subunit
• UniProt Protein Name: Integrin beta-7
• Protein Family: Integrin
• UniProt Gene Name: ITGB7
• UniProt Entry Name: ITB7_HUMAN

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms dimers with an alpha4 chain or an alphaE chain and plays a role in leukocyte adhesion. Dimerization with alpha4 forms a homing receptor for migration of lymphocytes to the intestinal mucosa and Peyer's patches. Dimerization with alphaE permits binding to the ligand epithelial cadherin, a calcium-dependent adhesion molecule. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Uniprot Description

ITGB7: integrin alpha-4/beta-7 (Peyer's patches-specific homing receptor lpam-1) is expected to play a role in adhesive interactions of leukocytes. It is a receptor for fibronectin and recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Integrin alpha-4/beta-7 is also a receptor for MADCAM1 and VCAM1. It recognizes the sequence L-D-T in MADCAM 1. Integrin alpha-e/beta-7 (HML-1) is a receptor for e-cadherin. Two alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex

Molecular Function: viral receptor activity; protein binding; metal ion binding; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; heterotypic cell-cell adhesion; regulation of immune response; entry of virus into host cell; extracellular matrix organization and biogenesis; cell-matrix adhesion; multicellular organismal development; cell adhesion; leukocyte tethering or rolling; receptor clustering