Goat anti-Human, Mouse Integrin, alpha9 Polyclonal Antibody | anti-ITGA9 antibody
Integrin, alpha9 (Integrin alpha4-like)
Gene Names
ITGA9; RLC; ITGA4L; ALPHA-RLC
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Integrin; alpha9; Polyclonal Antibody; alpha9 (Integrin alpha4-like); Anti -Integrin; anti-ITGA9 antibody
Host
Goat
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Integrin alpha9. Shows <5% crossreactivity with recombinant human Integrin alpha1, alpha8, alpha9, alphaL, recombinant mouse Integrin 3, 4, 5, E and M.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Applicable Applications for anti-ITGA9 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry and ELISA.
Dilution: Western Blot: 0.1ug/ml. Tested on recombinant mouse Integrin alpha9.
Flow Cytometry: 2.5ug labels 10e6 cells. Tested on D3 mouse embryonic stem cells.
Optimal dilutions should be determined by each laboratory for each application.
Dilution: Western Blot: 0.1ug/ml. Tested on recombinant mouse Integrin alpha9.
Flow Cytometry: 2.5ug labels 10e6 cells. Tested on D3 mouse embryonic stem cells.
Optimal dilutions should be determined by each laboratory for each application.
Immunogen
Recombinant protein corresponding to Try31-Val979 from mouse Integin alpha9 expressed in NSO cells (NP_598482).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-ITGA9 antibody
Integrin alpha9 is a 150kD type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta1 integrin. Alpha9beta1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse integrin alpha9 contains three beta-propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha9 ECD is absolutely conserved in mouse alpha9 ECD (Arg 566-Val 567). The ECD of mouse integrin alpha9 shares 95% and 89% aa sequence identity to rat and human integrin alpha9, respectively.
Product Categories/Family for anti-ITGA9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114,489 Da
NCBI Official Full Name
integrin alpha-9
NCBI Official Synonym Full Names
integrin, alpha 9
NCBI Official Symbol
ITGA9
NCBI Official Synonym Symbols
RLC; ITGA4L; ALPHA-RLC
NCBI Protein Information
integrin alpha-9; OTTHUMP00000161385; integrin alpha-RLC
UniProt Protein Name
Integrin alpha-9
Protein Family
UniProt Gene Name
ITGA9
UniProt Entry Name
ITA9_HUMAN
NCBI Description
This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers. [provided by RefSeq]
Uniprot Description
ITGA9: Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G- I-E-L in cytotactin. Belongs to the integrin alpha chain family.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: plasma membrane; basal plasma membrane; integrin complex
Molecular Function: collagen binding; metal ion binding; laminin binding
Biological Process: integrin-mediated signaling pathway; axon guidance; neutrophil chemotaxis; extracellular matrix organization and biogenesis; wound healing; cell adhesion
Research Articles on ITGA9
Similar Products
Product Notes
The ITGA9 itga9 (Catalog #AAA611310) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Integrin, alpha9 (Integrin alpha4-like) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin, alpha9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry and ELISA. Dilution: Western Blot: 0.1ug/ml. Tested on recombinant mouse Integrin alpha9. Flow Cytometry: 2.5ug labels 10e6 cells. Tested on D3 mouse embryonic stem cells. Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the ITGA9 itga9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin, alpha9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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