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Testing Data (Detection of human Integrin a9 with MBS606798. Lysates were prepared from human Tera2 cells in reducing sample buffer, resolved by SDS-PAGE (10ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2ug/ml MBS606798 and chemiluminescent detection substrate.)

Mouse anti-Human Integrin, alpha9 Monoclonal Antibody | anti-ITGA9 antibody

Integrin, alpha9 (Integrin, alpha4-like)

Gene Names
ITGA9; RLC; ITGA4L; ALPHA-RLC
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
Integrin; alpha9; Monoclonal Antibody; alpha9 (Integrin; alpha4-like); Anti -Integrin; anti-ITGA9 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9L835
Specificity
Recognizes human Integrin alpha 9. Species sequence homology: mouse (89%), rat (95%).
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-ITGA9 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Immunogen
Purified, E. coli-derived, recombinant human Integrin a9 (aa89-169 and aa501-557; Accession # Q13797).
Hybridoma
Mouse myeloma cells with B cells obtained from a mouse/rat immunized with purified, E. coli-derived, recombinant human Integrin a9 (aa89-169 and aa501-557).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Detection of human Integrin a9 with MBS606798. Lysates were prepared from human Tera2 cells in reducing sample buffer, resolved by SDS-PAGE (10ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2ug/ml MBS606798 and chemiluminescent detection substrate.)

Testing Data (Detection of human Integrin a9 with MBS606798. Lysates were prepared from human Tera2 cells in reducing sample buffer, resolved by SDS-PAGE (10ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2ug/ml MBS606798 and chemiluminescent detection substrate.)
Related Product Information for anti-ITGA9 antibody
Integrin alpha 9 is a 150kD type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the b1 Integrin. a9b1 binds to VEGF-C and D, and osteopontin. The 951aa extracellular domain of mouse Integrin a9 contains three b-propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human a9 ECD is absolutely conserved in mouse a9 ECD (Arg 566-Val 567). The ECD of mouse Integrin a9 shares 95% and 89% aa sequence identity to rat and human Integrin a9, respectively.
Product Categories/Family for anti-ITGA9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
114,489 Da
NCBI Official Full Name
integrin alpha-9
NCBI Official Synonym Full Names
integrin, alpha 9
NCBI Official Symbol
ITGA9
NCBI Official Synonym Symbols
RLC; ITGA4L; ALPHA-RLC
NCBI Protein Information
integrin alpha-9; OTTHUMP00000161385; integrin alpha-RLC
UniProt Protein Name
Integrin alpha-9
Protein Family
UniProt Gene Name
ITGA9
UniProt Entry Name
ITA9_HUMAN

NCBI Description

This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers. [provided by RefSeq]

Uniprot Description

ITGA9: Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G- I-E-L in cytotactin. Belongs to the integrin alpha chain family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: basal plasma membrane; plasma membrane; integrin complex

Molecular Function: collagen binding; metal ion binding; laminin binding

Biological Process: integrin-mediated signaling pathway; axon guidance; neutrophil chemotaxis; extracellular matrix organization and biogenesis; wound healing; cell adhesion

Research Articles on ITGA9

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Product Notes

The ITGA9 itga9 (Catalog #AAA606798) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Integrin, alpha9 (Integrin, alpha4-like) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin, alpha9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 2ug/ml. Researchers should empirically determine the suitability of the ITGA9 itga9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin, alpha9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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