Western Blot (WB)
(Western Blot: Sample: Recombinant Slit2, Human.)
Rabbit anti-Human Slit Homolog 2 (Slit2) Polyclonal Antibody | anti-Slit2 antibody
Polyclonal Antibody to Slit Homolog 2 (Slit2)
MGHHHHHHSGSEF-CQAIK CKHGKCRLSG LGQPYCECSS GYTGDSCDRE ISCRGERIRD YYQKQQGYAA CQTTKKVSRL ECRGGCAGGQ CCGPLRSKRR KYSFECTDGS SFVDEVEKVV KCGCTRCV
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant Slit2, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant Slit2, Human.)
Immunohistochemistry (IHC)
(HE staining on IHC-P; Samples: Human Colon Tissue)
Immunohistochemistry (IHC)
(HE staining on IHC-P; Samples: Human Colon Tissue)
Immunohistochemistry (IHC)
(HE staining on IHC-P; Samples: Human Pancreas Tissue.)
Immunohistochemistry (IHC)
(HE staining on IHC-P; Samples: Human Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4p15.31
Cellular Component: cytoplasm; extracellular region; extracellular space; membrane; plasma membrane
Molecular Function: calcium ion binding; GTPase inhibitor activity; heparin binding; identical protein binding; laminin-1 binding; protein binding; protein homodimerization activity; proteoglycan binding; Roundabout binding
Biological Process: axon extension involved in axon guidance; axon guidance; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cellular response to hormone stimulus; chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration; chemorepulsion involved in postnatal olfactory bulb interneuron migration; corticospinal neuron axon guidance through spinal cord; induction of negative chemotaxis; motor axon guidance; negative chemotaxis; negative regulation of actin filament polymerization; negative regulation of cell growth; negative regulation of cell migration; negative regulation of leukocyte chemotaxis; negative regulation of protein phosphorylation; negative regulation of small GTPase mediated signal transduction; negative regulation of smooth muscle cell migration; negative regulation of vascular permeability; positive regulation of apoptosis; positive regulation of axonogenesis; response to cortisol; retinal ganglion cell axon guidance; ureteric bud development
Research Articles on Slit2
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Product Notes
The Slit2 slit2 (Catalog #AAA2006224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Slit Homolog 2 (Slit2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Slit Homolog 2 (Slit2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the Slit2 slit2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-CQAIK CKHGKCRLSG LGQPYCECSS GYTGDSCDRE ISCRGERIRD YYQKQQGYAA CQTTKKVSRL ECRGGCAGGQ CCGPLRSKRR KYSFECTDGS SFVDEVEKVV KCGCTRCV. It is sometimes possible for the material contained within the vial of "Slit Homolog 2 (Slit2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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