Human Keratin 14 (KRT14) ELISA Kit | KRT14 elisa kit

Human Keratin 14 (KRT14) ELISA Kit

Cat. Num. AAA2019492

• Gene Names: KRT14; K14; NFJ; CK14; EBS3; EBS4
• Reactivity: Human
• Synonyms: Keratin 14 (KRT14); Human Keratin 14 (KRT14) ELISA Kit; CK14; EBS3; EBS4; K14; Cytokeratin 14; Epidermolysis Bullosa Simplex Dowling-Meara Koebner; Keratin; type I cytoskeletal 14; KRT14 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 14 (KRT14).; No significant cross-reactivity or interference between Keratin 14 (KRT14) and analogues was observed.
• Sequence Length: 472

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 14 (KRT14) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 14 (KRT14) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT14 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 14 (KRT14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 14 (KRT14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 14 (KRT14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 14 (KRT14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for KRT14 elisa kit

Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 15431310
• NCBI GeneID: 3861
• NCBI Accession #: NP_000517.2
• NCBI GenBank Nucleotide #: NM_000526.4
• UniProt Accession #: P02533; Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4
• Molecular Weight: 51,561 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 14
• NCBI Official Synonym Full Names: keratin 14, type I
• NCBI Official Symbol: KRT14
• NCBI Official Synonym Symbols: K14; NFJ; CK14; EBS3; EBS4
• NCBI Protein Information: keratin, type I cytoskeletal 14
• UniProt Protein Name: Keratin, type I cytoskeletal 14
• Protein Family: Keratin
• UniProt Gene Name: KRT14
• UniProt Synonym Gene Names: CK-14; K14
• UniProt Entry Name: K1C14_HUMAN

NCBI Description

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

Uniprot Description

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; keratin filament; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; aging; hair cycle

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type