nNOS active protein

nNOS (rat), (recombinant)

Cat. Num. AAA566095

• Gene Names: Nos1; bNOS
• Purity: >=90% (SDS-PAGE)
• Synonyms: nNOS; nNOS (rat); (recombinant); nNOS active protein

• Host: E Coli
• Purity/Purification: >=90% (SDS-PAGE)
• Form/Format: Liquid. In 50 mM TRIS, pH7.4, containing 0.1 mM EDTA, 10% glycerol, 100 mM NaCl, 0.1 mM DTT, 10uM tetrahydrobiopterin

• Source Note: Untagged rat nNOS
• Specific Activity: >=500U/mg. 1 U is defined as the amount of enzyme required to produce 1 nmol of NO (nitric o)xide per minute at 37 degree C. NO synthesis is measured by the conversion of oxyhemoglobin to methemoglobin
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Stable for at least 1 year after receipt when stored at -80 degree C. Keep stock vial on ice during experiments; Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for nNOS active protein

Nitric oxide synthases are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development. It may function as a retrograde neurotransmitter. Nitric oxide is mediated in mammals by the calcium-calmodulin controlled isoenzymes eNOS (endothelial NOS) and nNOS (neuronal NOS). The inducible isoform, iNOS, is involved in immune response, binds calmodulin at physiologically relevant concentrations, and produces NO as an immune defense mechanism, as NO is a free radical with an unpaired electron. It is the proximate cause of septic shock and may function in autoimmune disease.

Product Categories/Family for nNOS active protein

Cellular Analysis; Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 16258811
• NCBI GeneID: 24598
• NCBI Accession #: NP_434686.1
• NCBI GenBank Nucleotide #: NM_052799.1
• UniProt Accession #: P29476; P70594
• Molecular Weight: 164,430 Da
• NCBI Official Full Name: nitric oxide synthase, brain
• NCBI Official Synonym Full Names: nitric oxide synthase 1
• NCBI Official Symbol: Nos1
• NCBI Official Synonym Symbols: bNOS
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• UniProt Gene Name: Nos1
• UniProt Synonym Gene Names: Bnos; N-NOS; nNOS

NCBI Description

enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]

Uniprot Description

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.