Human Nitric oxide synthase, brain ELISA Kit | NOS1/nNOS elisa kit

Human Nitric oxide synthase, brain ELISA Kit

Cat. Num. AAA762959

• Gene Names: Nos1; bNOS
• Reactivity: Human
• Synonyms: Nitric oxide synthase; brain; Human Nitric oxide synthase; brain ELISA Kit; NOS1/bNOS/nNOS/Constitutive NOS/IHPS1/Neuronal NOS/nitric oxide synthase 1 (neuronal)/N-NOS/NOS type I/NOS/Peptidyl-cysteine S-nitrosylase NOS1; NOS1/nNOS elisa kit

• Reactivity: Human
• Sequence Length: 1463

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NOS1/nNOS elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human NOS1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NOS1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NOS1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NOS1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16258811
• NCBI GeneID: 24598
• NCBI Accession #: NP_434686.1
• NCBI GenBank Nucleotide #: NM_052799.1
• Molecular Weight: 164,430 Da
• NCBI Official Full Name: nitric oxide synthase, brain
• NCBI Official Synonym Full Names: nitric oxide synthase 1
• NCBI Official Symbol: Nos1
• NCBI Official Synonym Symbols: bNOS
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: Nos1
• UniProt Synonym Gene Names: Bnos; N-NOS; nNOS
• UniProt Entry Name: NOS1_RAT

NCBI Description

enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: Oxidoreductase; EC 1.14.13.39; Amino Acid Metabolism - arginine and proline

Cellular Component: azurophil granule; caveola; cytoplasm; cytoskeleton; cytosol; dendrite; dendritic spine; lipid raft; membrane; mitochondrial outer membrane; mitochondrion; nuclear membrane; nucleus; perinuclear region of cytoplasm; photoreceptor inner segment; plasma membrane; postsynaptic density; protein complex; sarcolemma; sarcoplasmic reticulum; sarcoplasmic reticulum membrane; synapse; T-tubule; vesicle membrane; Z disc

Molecular Function: amino acid binding; ATPase binding; cadmium ion binding; calmodulin binding; enzyme binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding; protein homodimerization activity; sodium channel regulator activity

Biological Process: aging; arginine catabolic process; behavioral response to cocaine; exogenous drug catabolic process; female pregnancy; multicellular organismal response to stress; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of heart contraction; negative regulation of hydrolase activity; negative regulation of insulin secretion; negative regulation of peptidyl-serine phosphorylation; negative regulation of potassium ion transport; negative regulation of serotonin uptake; negative regulation of vasoconstriction; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; reduction of cytosolic calcium ion concentration; regulation of heart contraction; regulation of neurogenesis; regulation of sensory perception of pain; regulation of sodium ion transport; response to activity; response to estrogen stimulus; response to ethanol; response to heat; response to hypoxia; response to lead ion; response to lipopolysaccharide; response to nicotine; response to nutrient levels; response to organic cyclic substance; response to organic nitrogen; response to peptide hormone stimulus; response to vitamin E; striated muscle contraction