Rabbit anti-Mouse, Rat nNOS Polyclonal Antibody | anti-nNOS antibody

nNOS polyclonal antibody

Cat. Num. AAA565720

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: nNOS; Polyclonal Antibody; nNOS polyclonal antibody; Nitric oxide synthase (neuronal); NOS I; pAb; anti-nNOS antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes mouse and rat nNOS. Detects a band of ~155kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 1434

• Applicable Applications for anti-nNOS antibody: Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Western Blot (1:250, ECL).; Detects a band of ~155kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of human nNOS.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis: Lane 1: MW marker, Lane 2: Rat Brain, Lane 3: Mouse Brain.)

Immunohistochemistry (IHC)

(IHC Analysis: Human brain, cortex tissue stained with nNOS, pAb at 20µg/ml.)

Related Product Information for anti-nNOS antibody

The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.

Product Categories/Family for anti-nNOS antibody

Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 1709333
• NCBI GeneID: 4842
• UniProt Accession #: P29475; O75713; E9PH30
• Molecular Weight: 164,779 Da
• NCBI Official Full Name: Nitric oxide synthase, brain
• NCBI Official Synonym Full Names: nitric oxide synthase 1
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS

NCBI Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: cytoplasm; cytoskeleton; cytosol; lipid raft; mitochondrion; perinuclear region of cytoplasm; photoreceptor inner segment; protein complex; sarcolemma; sarcoplasmic reticulum; synapse

Molecular Function: cadmium ion binding; FAD binding; FMN binding; heme binding; NADP binding; nitric-oxide synthase activity; protein binding; sodium channel regulator activity

Biological Process: arginine catabolic process; cell redox homeostasis; exogenous drug catabolic process; multicellular organismal response to stress; myoblast fusion; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of hydrolase activity; negative regulation of potassium ion transport; negative regulation of serotonin uptake; neurotransmitter biosynthetic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; regulation of sodium ion transport; response to heat; response to hypoxia; striated muscle contraction

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1