Rat Nitric oxide synthase, brain ELISA Kit | Nos1 elisa kit

Rat Nitric oxide synthase, brain ELISA Kit

Cat. Num. AAA2884948

• Gene Names: Nos1; bNOS
• Reactivity: Rat
• Synonyms: Nitric oxide synthase; brain; Rat Nitric oxide synthase; brain ELISA Kit; BNOS; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; Nos1; Bnos; 1.14.13.39; Nos1 elisa kit

• Reactivity: Rat
• Sequence Length: 1463

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Nos1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16258811
• NCBI GeneID: 24598
• NCBI Accession #: NP_434686.1
• NCBI GenBank Nucleotide #: NM_052799.1
• UniProt Accession #: P29476; P70594
• Molecular Weight: 164,430 Da
• NCBI Official Full Name: nitric oxide synthase, brain
• NCBI Official Synonym Full Names: nitric oxide synthase 1
• NCBI Official Symbol: Nos1
• NCBI Official Synonym Symbols: bNOS
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: Nos1
• UniProt Synonym Gene Names: Bnos; N-NOS; nNOS
• UniProt Entry Name: NOS1_RAT

NCBI Description

enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Cellular Component: azurophil granule; caveola; cytoplasm; cytoskeleton; cytosol; dendrite; dendritic spine; lipid raft; membrane; mitochondrial outer membrane; mitochondrion; nuclear membrane; nucleus; perinuclear region of cytoplasm; photoreceptor inner segment; plasma membrane; postsynaptic density; protein complex; sarcolemma; sarcoplasmic reticulum; sarcoplasmic reticulum membrane; synapse; T-tubule; vesicle membrane; Z disc

Molecular Function: amino acid binding; ATPase binding; cadmium ion binding; calmodulin binding; enzyme binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding; protein homodimerization activity; sodium channel regulator activity

Biological Process: aging; arginine catabolic process; behavioral response to cocaine; exogenous drug catabolic process; female pregnancy; multicellular organismal response to stress; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of heart contraction; negative regulation of hydrolase activity; negative regulation of insulin secretion; negative regulation of peptidyl-serine phosphorylation; negative regulation of potassium ion transport; negative regulation of serotonin uptake; negative regulation of vasoconstriction; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; reduction of cytosolic calcium ion concentration; regulation of heart contraction; regulation of neurogenesis; regulation of sensory perception of pain; regulation of sodium ion transport; response to activity; response to estrogen stimulus; response to ethanol; response to heat; response to hypoxia; response to lead ion; response to lipopolysaccharide; response to nicotine; response to nutrient levels; response to organic cyclic substance; response to organic nitrogen; response to peptide hormone stimulus; response to vitamin E; striated muscle contraction