Mouse NOS1/nNOS Monoclonal Antibody | anti-NOS1/nNOS antibody

NOS1/nNOS Antibody: RPE

Cat. Num. AAA8005888

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, ELISA, Immunohistochemistry
• Purity: Protein A Purified
• Synonyms: NOS1/nNOS; Monoclonal Antibody; NOS1/nNOS Antibody: RPE; Mouse Anti-Human NOS1 Monoclonal IgG2a; BNOS Antibody; Constitutive NOS Antibody; IHPS 1 Antibody; IHPS1 Antibody; N-NOS Antibody; NC-NOS Antibody; neuronal Nitric Oxide Synthase Antibody; Neuronal NOS Antibody; Nitric oxide synthase neuronal included Antibody; Nitric oxide synthase 1 (neuronal) Antibody; Nitric oxide synthase 1 Antibody; NNOS Antibody; NO Antibody; NOS 1 Antibody; NOS Antibody; NOS type I Antibody; NOS-I Antibody; NOS1 Antibody; NOS1_HUMAN Antibody; anti-NOS1/nNOS antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: J23
• Specificity: Detects ~160.97kDa.
• Purity/Purification: Protein A Purified
• Form/Format: PBS pH7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NOS1/nNOS antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000; A 1:1000 dilution was sufficient for detection of NOS1/Nnos in 7.5ug of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG: HRP as the secondary antibody.
• Immunogen: Partial Human Recombinant NOS1 Protein
• Immunogen Species: Human
• Conjugation: RPE
• Tissue Specificity: Ubiquitously expressed; detected in skeletal muscle and brain, also in testis, lung and kidney.
• Cellular Localization: Cell Membrane
• Preparation and Storage: Store at -20 degree C; Shipping: Blue Ice

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NOS1/nNos Monoclonal Antibody, Clone J23 . Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-NOS1/nNos Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) NOS1/nNOS Antibody. (D) Hoechst (E) Composite.)

Western Blot (WB)

(Western Blot analysis of Mouse Cerebellum showing detection of NOS1/nNos protein using Mouse Anti-NOS1/nNos Monoclonal Antibody, Clone J23 . Lane 1: MW ladder. Lane 2: Mouse Brain Cerebellum (7.5 ug). Load: 7.5 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-NOS1/nNos Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

Related Product Information for anti-NOS1/nNOS antibody

nNOS/NOS I, also known as NC-NOS, or NOS type I, or Neuronal NOS, or produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. There are two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II).

Product Categories/Family for anti-NOS1/nNOS antibody

Antibodies; Neuroscience; Neurotransmission; Nitric Oxide; Cancer; Cancer Metabolism; Response to Hypoxia

NCBI and Uniprot Product Information

• NCBI GI #: 10835173
• NCBI GeneID: 4842
• NCBI Accession #: NP_000611.1
• NCBI GenBank Nucleotide #: NM_000620.4
• UniProt Accession #: P29475; O75713; E9PH30
• Molecular Weight: 160,970 Da
• NCBI Official Full Name: nitric oxide synthase, brain isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 1 (neuronal)
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1
• UniProt Protein Name: Nitric oxide synthase, brain
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: photoreceptor inner segment; protein complex; cytoskeleton; sarcoplasmic reticulum; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft

Molecular Function: calmodulin binding; protein binding; sodium channel regulator activity; FAD binding; NADPH-hemoprotein reductase activity; FMN binding; nitric-oxide synthase activity; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding

Biological Process: interaction with host; negative regulation of serotonin uptake; negative regulation of calcium ion transport; striated muscle contraction; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of blood pressure; nitric oxide biosynthetic process; blood coagulation; nitric oxide mediated signal transduction; multicellular organismal response to stress; myoblast fusion; neurotransmitter biosynthetic process

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1