Rabbit NOS1 / nNOS Polyclonal Antibody | anti-NOS1 / nNOS antibody

Anti-NOS1 / nNOS Antibody (aa818-867) IHC-plus

Cat. Num. AAA2400270

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: NOS1 / nNOS; Polyclonal Antibody; Anti-NOS1 / nNOS Antibody (aa818-867) IHC-plus; Rabbit Polyclonal (IgG) to Human NOS1 / nNOS; Human NOS1 / nNOS; BNOS; Constitutive NOS; IHPS1; NNOS; NC-NOS; Nitric oxide synthase; brain; N-NOS; Neuronal NOS; NOS; NOS type I; Nos; type i; anti-NOS1 / nNOS antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: nNOS Antibody detects endogenous levels of total nNOS protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1434

• Applicable Applications for anti-NOS1 / nNOS antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000); IF (1:100 - 1:500); IHC-P (20 ug/ml); WB (1:500 - 1:1000)
• Immunogen: NOS1 / nNOS antibody was raised against synthetic peptide from human nNOS (aa818-867).
• Immunogen Description: Synthetic peptide from human nNOS (aa818-867).
• Antigen Modification: aa818-867
• Target Species: Human
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-NOS1 / nNOS antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells, using nNOS (Ab-852) Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from Raw264.7 cells, treated with INF 2500u/ml 10', using nNOS (Ab-852) Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-NOS1 / nNOS antibody

NOS1 Antibody, BNOS Antibody, Constitutive NOS Antibody, IHPS1 Antibody, NNOS Antibody, NC-NOS Antibody, Nitric oxide synthase, brain Antibody, N-NOS Antibody, Neuronal NOS Antibody, NOS Antibody, NOS type I Antibody, Nos, type i Antibody Description: NOS1 / nNOS belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities.

NCBI and Uniprot Product Information

• NCBI GI #: 10835173
• NCBI GeneID: 4842
• NCBI Accession #: NP_000611.1
• NCBI GenBank Nucleotide #: NM_000620.4
• UniProt Accession #: P29475; O75713; E9PH30
• Molecular Weight: 164,779 Da
• NCBI Official Full Name: nitric oxide synthase, brain isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 1
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: azurophil granule; caveola; cytoplasm; cytoskeleton; cytosol; dendritic spine; lipid raft; mitochondrial outer membrane; mitochondrion; nuclear membrane; perinuclear region of cytoplasm; photoreceptor inner segment; postsynaptic density; protein complex; sarcolemma; sarcoplasmic reticulum; synapse; T-tubule; vesicle membrane; Z disc

Molecular Function: cadmium ion binding; calmodulin binding; enzyme binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding; protein homodimerization activity; sodium channel regulator activity

Biological Process: aging; arginine catabolic process; behavioral response to cocaine; blood coagulation; cell redox homeostasis; exogenous drug catabolic process; female pregnancy; multicellular organismal response to stress; myoblast fusion; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of heart contraction; negative regulation of hydrolase activity; negative regulation of insulin secretion; negative regulation of potassium ion transport; negative regulation of serotonin uptake; negative regulation of vasoconstriction; neurotransmitter biosynthetic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; reduction of cytosolic calcium ion concentration; regulation of neurogenesis; regulation of sensory perception of pain; regulation of sodium ion transport; response to activity; response to estrogen stimulus; response to ethanol; response to heat; response to hypoxia; response to lead ion; response to lipopolysaccharide; response to nicotine; response to peptide hormone stimulus; response to vitamin E; striated muscle contraction

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1