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Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using nNOS antibody)

Rabbit nNOS Polyclonal Antibody | anti-nNOS antibody

nNOS antibody

Applications
Immunofluorescence
Purity
Purified by immunoaffinity chromatography.
Synonyms
nNOS; Polyclonal Antibody; nNOS antibody; Rabbit polyclonal nNOS antibody; Polyclonal nNOS antibody; Anti-nNOS antibody; Ab 852 antibody; Nitric oxide synthases antibody; anti-nNOS antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-nNOS antibody
Immunofluorescence (IF)
Application Notes
IF: 1:100-1:200
Immunogen
nNOS antibody was raised in Rabbit using the peptide sequence around aa 850~854 (F-N-S-V-S) derived from Human nNOS as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using nNOS antibody)

Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using nNOS antibody)

Western Blot (WB)

(Western Blot analysis of extracts from Rat and Mouse brain tissue using nNOS antibody)

Western Blot (WB) (Western Blot analysis of extracts from Rat and Mouse brain tissue using nNOS antibody)
Related Product Information for anti-nNOS antibody
NOS are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule, having a vital role in many biological processes. It is the intercellular signal that controls vascular tone (hence blood pressure), insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis (growth of new blood vessels) and in the development of nervous system. It is believed to function as a retrograde neurotransmitter and hence is likely to be important in learning. Nitric oxide signalling is mediated in mammals by the calcium/calmodulin controlled isoenzymes eNOS (endothelial NOS) and nNOS (neuronal NOS); the inducible isoform iNOS is involved in immune response, binds calmodulin at all physiologically relevant concentrations, and produces large amounts of NO as a defense mechanism.
Product Categories/Family for anti-nNOS antibody

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Product Notes

The nNOS (Catalog #AAA5309734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's nNOS can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). IF: 1:100-1:200. Researchers should empirically determine the suitability of the nNOS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "nNOS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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