Western Blot (WB)
(Figure: Western blot analysis of the time course of assembly of the inflammasome using MAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages.Method: Assembly of the inflammasome was induced by shifting the temperature to 30 degree C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated using PAb to Asc (AL177) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) at 1:1'000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5'000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon; Mol. Cell. 10, 417 (2002).)
NLRP1/NALP1 Monoclonal Antibody | anti-NLRP1/NALP1 antibody
NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4)
Does not cross-react with mouse NLRP1/NALP1.
Does not cross-react with mouse NLRP1/NALP1.
Immunohistochemistry (paraffin sections, microwave treatment)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Short term: 4 degree C
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 degree C.
Western Blot (WB)
(Figure: Western blot analysis of the time course of assembly of the inflammasome using MAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages.Method: Assembly of the inflammasome was induced by shifting the temperature to 30 degree C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated using PAb to Asc (AL177) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) at 1:1'000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5'000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon; Mol. Cell. 10, 417 (2002).)
Western Blot (WB)
(Figure: Western blot analysis of the time course of assembly of the inflammasome using MAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages.Method: Assembly of the inflammasome was induced by shifting the temperature to 30 degree C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated using PAb to Asc (AL177) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) at 1:1'000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5'000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon; Mol. Cell. 10, 417 (2002).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP1, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PubMed:22665479, PubMed:17418785). May be activated by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, in a NOD2-dependent manner (PubMed:18511561). Contrary to its mouse ortholog, not activated by Bacillus anthracis lethal toxin (PubMed:19651869). It is unclear whether isoform 2 is involved in inflammasome formation. It is not cleaved within the FIIND domain, does not assemble into specks, nor promote IL1B release (PubMed:22665479). However, in an vitro cell-free system, it has been shown to be activated by MDP (PubMed:17349957). Binds ATP (PubMed:11113115, PubMed:15212762).
Research Articles on NLRP1/NALP1
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Product Notes
The NLRP1/NALP1 nlrp1 (Catalog #AAA565136) is an Antibody produced from Mouse: Purified from concentrated hybridoma tissue culture supernatant. and is intended for research purposes only. The product is available for immediate purchase. The NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4) reacts with Human Does not cross-react with mouse NLRP1/NALP1. and may cross-react with other species as described in the data sheet. AAA Biotech's NLRP1/NALP1 can be used in a range of immunoassay formats including, but not limited to, Immunocyotochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB). Immunocytochemistry (1:1,000) Immunohistochemistry (paraffin sections, microwave treatment) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Researchers should empirically determine the suitability of the NLRP1/NALP1 nlrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NLRP1/NALP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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