Rabbit anti-Human NALP1 Polyclonal Antibody | anti-NALP1 antibody

NALP1 Antibody

Cat. Num. AAA9410009

• Gene Names: NLRP1; NAC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Immunoaffinity chromatography purified IgG
• Synonyms: NALP1; Polyclonal Antibody; NALP1 Antibody; CARD7; DEFCAP; NACHT domain-leucine-rich-repeat and PYD-containing protein 1; anti-NALP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity chromatography purified IgG
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 1429

• Applicable Applications for anti-NALP1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1.
• Target Name: NALP1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 ug/mL.)

Related Product Information for anti-NALP1 antibody

NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.

Product Categories/Family for anti-NALP1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 11096303
• NCBI GeneID: 22861
• Molecular Weight: 46,068 Da
• NCBI Official Full Name: NALP1
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 1
• NCBI Official Symbol: NLRP1
• NCBI Official Synonym Symbols: NAC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 1
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 1
• UniProt Gene Name: NLRP1
• UniProt Synonym Gene Names: CARD7; DEFCAP; KIAA0926; NAC; NALP1
• UniProt Entry Name: NALP1_HUMAN

NCBI Description

This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

NALP1: Able to form cytoplasmic structures termed death effector filaments. Enhances APAF1 and cytochrome c-dependent activation of pro-caspase-9 and consecutive apoptosis. Stimulates apoptosis through activation of caspase-3. Involved in activation of caspase-1 and caspase-5 as part of the NALP1 inflammasome complex which leads to processing and release of IL1B and IL18. Binds ATP. Interacts strongly with caspase-2, weakly with caspase-9 and with APAF1 in a cytochrome c-inducible way, leading to the formation of an apoptosome. This interaction may be ATP-dependent. Part of the NALP1 inflammasome complex which is involved in activation of caspase-1 and caspase-5, leading to processing of IL1B and IL18. The complex is activated by bacterial muramyl dipeptide which triggers ATP-binding and oligomerization of NALP1. Widely expressed. Isoform 1 and isoform 2 are expressed in peripheral blood leukocytes and chronic myelogenous leukemia cell line K-562, followed by thymus, spleen and heart. Also detected in brain, lung, placenta, small intestine, colon, kidney, liver, muscle, testis and epithelial cells. Absent from hematopoietic progenitor cells but expressed upon differentiation of cells into granulocytes and, to a lesser extent, monocytes. In peripheral blood cells, highest levels are found in T-lymphocytes, granulocytes and monocytes. Expression is significantly increased in bone marrow blast cells of some acute leukemia patients but not in solid tumors. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: cytosol; intracellular; nucleus

Molecular Function: ATP binding; caspase activator activity; enzyme binding; protein binding; protein domain specific binding

Biological Process: apoptosis; caspase activation; defense response to bacterium; inflammatory response; innate immune response; neuron apoptosis; positive regulation of interleukin-1 beta secretion; regulation of inflammatory response; response to muramyl dipeptide

Disease: Corneal Intraepithelial Dyskeratosis And Ectodermal Dysplasia; Vitiligo-associated Multiple Autoimmune Disease Susceptibility 1