Western Blot (WB)
(Fig: 1 Western blot analysis of NALP1 (NAC/CARD7) in Jurkat cells using the NALP1 antibody at 1: 2000.)
Rabbit anti-Human NLRP1/NALP1 Polyclonal Antibody | anti-NLRP1 antibody
Polyclonal Antibody to NLRP1/NALP1 (NAC-alpha)
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig: 1 Western blot analysis of NALP1 (NAC/CARD7) in Jurkat cells using the NALP1 antibody at 1: 2000.)
Western Blot (WB)
(Fig: 1 Western blot analysis of NALP1 (NAC/CARD7) in Jurkat cells using the NALP1 antibody at 1: 2000.)
Immunohistochemistry (IHC)
(Fig: 2 Formalin-fixed, paraffin-embedded normal human bone marrow stained for NALP1 (CARD7/NAP) using NALP1 antibody at 1: 2000. Hematoxylin-eosin counterstain. Staining is seen in differentiated granulocytes.)
Immunohistochemistry (IHC)
(Fig: 2 Formalin-fixed, paraffin-embedded normal human bone marrow stained for NALP1 (CARD7/NAP) using NALP1 antibody at 1: 2000. Hematoxylin-eosin counterstain. Staining is seen in differentiated granulocytes.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP1, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PubMed:22665479, PubMed:17418785). May be activated by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, in a NOD2-dependent manner (PubMed:18511561). Contrary to its mouse ortholog, not activated by Bacillus anthracis lethal toxin (PubMed:19651869). It is unclear whether isoform 2 is involved in inflammasome formation. It is not cleaved within the FIIND domain, does not assemble into specks, nor promote IL1B release (PubMed:22665479). However, in an vitro cell-free system, it has been shown to be activated by MDP (PubMed:17349957). Binds ATP (PubMed:11113115, PubMed:15212762).
Research Articles on NLRP1
Similar Products
Product Notes
The NLRP1 nlrp1 (Catalog #AAA668732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to NLRP1/NALP1 (NAC-alpha) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NLRP1/NALP1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB). WB: 1: 1000-1: 2000 IHC (paraffin): 1: 1000-1: 5000 IP: 1: 50-1: 200. Researchers should empirically determine the suitability of the NLRP1 nlrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NLRP1/NALP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
