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Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
Rabbit anti-Human Pericentriolar Material 1 Protein Polyclonal Antibody | anti-PCM1 antibody
Pericentriolar Material 1 Protein (PCM1, PCM-1, hPCM-1, PTC4) (MaxLight 550)
Gene Names
PCM1; PTC4
Reactivity
Human
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
Pericentriolar Material 1 Protein; Polyclonal Antibody; Pericentriolar Material 1 Protein (PCM1; PCM-1; hPCM-1; PTC4) (MaxLight 550); anti-PCM1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PCM-1.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-PCM1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human PCM-1 (KLH).
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
Western Blot (WB)
(Western blot analysis of PCM-1 antibody (Center) in Ramos cell line lysates (35ug/lane). PCM-1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PCM-1 antibody (Center) in Ramos cell line lysates (35ug/lane). PCM-1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PCM-1 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PCM-1 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-PCM1 antibody
References
1. Balczon R., Bao L.J. Cell Biol. 124:783-793(1994). 2. Corvi R., Berger N.Oncogene 19:4236-4242(2000). 3. Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002). 4. Reiter A., Walz C.Cancer Res. 65:2662-2667(2005). 5. Murati A., Gelsi-Boyer V.Leukemia 19:1692-1696(2005). 6. Hames R.S., Crookes R.E.Mol. Biol. Cell 16:1711-1724(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,199 Da
NCBI Official Full Name
pericentriolar material 1 protein
NCBI Official Synonym Full Names
pericentriolar material 1
NCBI Official Symbol
PCM1
NCBI Official Synonym Symbols
PTC4
NCBI Protein Information
pericentriolar material 1 protein; PCM-1; hPCM-1; pericentriolar material 1, PCM1
UniProt Protein Name
Pericentriolar material 1 protein
Protein Family
UniProt Gene Name
PCM1
UniProt Synonym Gene Names
PCM-1; hPCM-1
UniProt Entry Name
PCM1_HUMAN
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Product Notes
The PCM1 pcm1 (Catalog #AAA6492687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pericentriolar Material 1 Protein (PCM1, PCM-1, hPCM-1, PTC4) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pericentriolar Material 1 Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PCM1 pcm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pericentriolar Material 1 Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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