Rabbit anti-Human NALP1 / NLRP1 Polyclonal Antibody | anti-NALP1 antibody

Rabbit Polyclonal to Human NALP1 / NLRP1

Cat. Num. AAA240330

• Gene Names: NLRP1; NAC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Purified IgG
• Synonyms: NALP1 / NLRP1; Polyclonal Antibody; Rabbit Polyclonal to Human NALP1 / NLRP1; Anti-NALP1 / NLRP1 Antibody (C-Terminus) IHC-plus; NLRP1; CLR17.1; CARD7; DEFCAP; DEFCAP-L/S; NAC; SLEV1; VAMAS1; KIAA0926; NALP1; PP1044; Human NALP1; anti-NALP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: peptide corresponding to 13 amino acids near the carboxy-terminus of human NALP1
• Purity/Purification: Purified IgG
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1429

• Applicable Applications for anti-NALP1 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC, IHC-P (2.5 ug/ml), WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human NLRP1 / NALP1.
• Immunogen Type: Synthetic peptide
• Immunogen: NALP1 / NLRP1 antibody was raised against synthetic peptide from human NLRP1 / NALP1.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-NLRP1 / NALP1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of NALP1 in K562 cells with NALP1 antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 7662386
• NCBI GeneID: 22861
• NCBI Accession #: NP_055737.1
• NCBI GenBank Nucleotide #: NM_014922.4
• UniProt Accession #: Q9C000; Q9BZZ8; Q9BZZ9; Q9H5Z7; Q9H5Z8; Q9HAV8; Q9UFT4; Q9Y2E0; E9PE50; I6L9D9
• Molecular Weight: 46,068 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 1
• NCBI Official Symbol: NLRP1
• NCBI Official Synonym Symbols: NAC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 1; caspase recruitment domain protein 7; NACHT, LRR and PYD containing protein 1; NACHT, leucine rich repeat and PYD containing 1; caspase recruitment domain-containing protein 7; nucleotide-binding domain and caspase recruitment domain; death effector filament-forming Ced-4-like apoptosis protein; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 1
• UniProt Gene Name: NLRP1
• UniProt Synonym Gene Names: CARD7; DEFCAP; KIAA0926; NAC; NALP1
• UniProt Entry Name: NALP1_HUMAN

NCBI Description

This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

NALP1: Able to form cytoplasmic structures termed death effector filaments. Enhances APAF1 and cytochrome c-dependent activation of pro-caspase-9 and consecutive apoptosis. Stimulates apoptosis through activation of caspase-3. Involved in activation of caspase-1 and caspase-5 as part of the NALP1 inflammasome complex which leads to processing and release of IL1B and IL18. Binds ATP. Interacts strongly with caspase-2, weakly with caspase-9 and with APAF1 in a cytochrome c-inducible way, leading to the formation of an apoptosome. This interaction may be ATP-dependent. Part of the NALP1 inflammasome complex which is involved in activation of caspase-1 and caspase-5, leading to processing of IL1B and IL18. The complex is activated by bacterial muramyl dipeptide which triggers ATP-binding and oligomerization of NALP1. Widely expressed. Isoform 1 and isoform 2 are expressed in peripheral blood leukocytes and chronic myelogenous leukemia cell line K-562, followed by thymus, spleen and heart. Also detected in brain, lung, placenta, small intestine, colon, kidney, liver, muscle, testis and epithelial cells. Absent from hematopoietic progenitor cells but expressed upon differentiation of cells into granulocytes and, to a lesser extent, monocytes. In peripheral blood cells, highest levels are found in T-lymphocytes, granulocytes and monocytes. Expression is significantly increased in bone marrow blast cells of some acute leukemia patients but not in solid tumors. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: intracellular; cytosol; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; caspase activator activity; ATP binding

Biological Process: caspase activation; neuron apoptosis; apoptosis; response to muramyl dipeptide; regulation of inflammatory response; defense response to bacterium; innate immune response; positive regulation of interleukin-1 beta secretion

Disease: Vitiligo-associated Multiple Autoimmune Disease Susceptibility 1; Corneal Intraepithelial Dyskeratosis And Ectodermal Dysplasia