Testing Data
HEMK1 recombinant protein
HEMK1 Protein
Gene Names
HEMK1; HEMK; MTQ1
Applications
Methyltransferase Assay
Synonyms
HEMK1; HEMK1 Protein; HEMK1 recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full-length
Sequence Length
6203
Applicable Applications for HEMK1 recombinant protein
Methyltransferase Assay
Species
Human
Tag
GST tag
Testing Data
Testing Data
Related Product Information for HEMK1 recombinant protein
Description: Recombinant full-length human HEMK1 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: HEMK1 belongs to the HemK methyltransferase family, members of which are found in genomes from bacteria to humans (1). HEMK1 methylates the glutamine residue in the GGQ motif of HMRF1L, a human mitochondrial release factor, and plays an important regulatory role in translation termination process (2).
Scientific Background: HEMK1 belongs to the HemK methyltransferase family, members of which are found in genomes from bacteria to humans (1). HEMK1 methylates the glutamine residue in the GGQ motif of HMRF1L, a human mitochondrial release factor, and plays an important regulatory role in translation termination process (2).
Product Categories/Family for HEMK1 recombinant protein
References
1. Bujnicki, J.M., et al. Is the HemK family of putative S-adenosylmethionine-dependent methyltransferases a "missing" zeta subfamily of adenine methyltransferases? A hypothesis. IUBMB Life. 48 (3):247-9, 1999. 2. Shizawa, T., et al. The human mitochondrial translation release factor HMRF1L is methylated in the GGQ motif by the methyltransferase HMPrmC. Biochem Biophys Res Commun. 373 (1):99-103, 2008.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65 kDa
NCBI Official Full Name
Homo sapiens HemK methyltransferase family member 1 (HEMK1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
HemK methyltransferase family member 1
NCBI Official Symbol
HEMK1
NCBI Official Synonym Symbols
HEMK; MTQ1
NCBI Protein Information
hemK methyltransferase family member 1
UniProt Protein Name
HemK methyltransferase family member 1
Protein Family
UniProt Gene Name
HEMK1
UniProt Synonym Gene Names
HEMK
Uniprot Description
HEMK1: N5-glutamine methyltransferase responsible for the methylation of the GGQ triplet of the mitochondrial translation release factor MTRF1L. Belongs to the protein N5-glutamine methyltransferase family.
Protein type: Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; EC 2.1.1.-; Lipid Metabolism - androgen and estrogen; Methyltransferase; Other Amino Acids Metabolism - selenoamino acid
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: mitochondrion
Molecular Function: protein binding; S-adenosylmethionine-dependent methyltransferase activity
Research Articles on HEMK1
Similar Products
Product Notes
The HEMK1 hemk1 (Catalog #AAA515508) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full-length. AAA Biotech's HEMK1 can be used in a range of immunoassay formats including, but not limited to, Methyltransferase Assay. Researchers should empirically determine the suitability of the HEMK1 hemk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEMK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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