Testing Data
PCMT1 recombinant protein
PCMT1 Protein
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Scientific Background: PCMT1 is a member of the L-isoaspartyl/D-aspartyl protein methyltransferase family, which plays an important role in protein repair (1). Downregulation of PCMT1 leads to hyperactivation of MAPK pathway upon EGF stimulation (2). PCMT1 is regulated by cell-matrix interactions, and linked to various biological processes including wound healing, cell migration, and tumor metastasis (3).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
PCMT1: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.1.1.77; Methyltransferase
Chromosomal Location of Human Ortholog: 6q25.1
Cellular Component: cytoplasm; cytosol
Molecular Function: cadherin binding; protein binding; protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
Biological Process: protein amino acid methylation; protein repair
Research Articles on PCMT1
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Product Notes
The PCMT1 pcmt1 (Catalog #AAA515595) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-end. AAA Biotech's PCMT1 can be used in a range of immunoassay formats including, but not limited to, Methyltransferase Assay. Researchers should empirically determine the suitability of the PCMT1 pcmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCMT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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