Rabbit anti-Human ARSF Polyclonal Antibody | anti-ARSF antibody

ARSF, ID (ARSF, Arylsulfatase F) (Biotin)

Cat. Num. AAA6275415

• Gene Names: ARSF; ASF
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARSF; Polyclonal Antibody; ID (ARSF; Arylsulfatase F) (Biotin); Arylsulfatase F; anti-ARSF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ARSF antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ARSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from the Central region of human ARSF.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARSF Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ARSF antibody detected the ARSF protein (arrow).)

Western Blot (WB)

(Western blot analysis of ARSF (arrow) using rabbit polyclonal ARSF Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARSF gene.)

Flow Cytometry (FC/FACS)

(ARSF Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ARSF antibody

This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin.

Product Categories/Family for anti-ARSF antibody

Antibodies; Disease Markers

References

Cao, W., et al. J. Biol. Chem. 273(32):20629-20635(1998). Puca, A.A., et al. Genomics 42(2):192-199(1997). Parenti, G., et al. Curr. Opin. Genet. Dev. 7(3):386-391(1997). Franco, B., et al. Cell 81(1):15-25(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 31742482
• NCBI GeneID: 416
• NCBI Accession #: NP_004033.2
• NCBI GenBank Nucleotide #: NM_004042.4
• UniProt Accession #: P54793; Q8TCC5
• Molecular Weight: 65,940 Da
• NCBI Official Full Name: arylsulfatase F
• NCBI Official Synonym Full Names: arylsulfatase F
• NCBI Official Symbol: ARSF
• NCBI Official Synonym Symbols: ASF
• NCBI Protein Information: arylsulfatase F
• UniProt Protein Name: Arylsulfatase F
• Protein Family: Arylsulfatase
• UniProt Gene Name: ARSF
• UniProt Synonym Gene Names: ASF
• UniProt Entry Name: ARSF_HUMAN

NCBI Description

This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]

Uniprot Description

ARSF: a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]

Protein type: Secreted; EC 3.1.6.-; Secreted, signal peptide; Hydrolase

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: endoplasmic reticulum lumen

Molecular Function: arylsulfatase activity; metal ion binding

Biological Process: sphingolipid metabolic process; cellular protein metabolic process; glycosphingolipid metabolic process; post-translational protein modification