Immunohistochemistry (IHC)
(Immunochemical staining of human HEMK1 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human HEMK1 Polyclonal Antibody | anti-HEMK1 antibody
Anti-HEMK1 Antibody, Rabbit Polyclonal
Gene Names
HEMK1; HEMK; MTQ1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
HEMK1; Polyclonal Antibody; Anti-HEMK1 Antibody; Rabbit Polyclonal; HEMK1 Antibody; Rabbit PAb; Antigen Affinity Purified; HemK methyltransferase family member 1; Anti-HEMK Antibody; Anti-MTQ1 Antibody; anti-HEMK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human HEMK1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-HEMK1 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-1:400
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human HEMK1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HEMK1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human HEMK1 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human HEMK1 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-HEMK1 antibody
HEMK1 (HemK Methyltransferase Family Member 1) is a protein that in humans is encoded by the HEMK1 gene. The HEMK1 gene, located on 3p21.31, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, and frog. An important paralog of this gene is N6AMT1. 243 organisms have orthologs with the human gene HEMK1. HEMK1 is widely expressed in the thyroid, ovary, and other tissues. HEMK1 belongs to the protein N5-glutamine methyltransferase family, which is responsible for the methylation of the glutamine residue in the universally conserved GGQ motif of the mitochondrial translation release factor MTRF1L.
References
Rolland T, et al. (2014) A proteome-scale map of the human interactome network. Cell 159 (5): 1212-1226.Ishizawa T, et al. (2008) The human mitochondrial translation release factor hmrf1l is methylated in the ggq motif by the methyltransferase hmprmc. Biochem Biophys Res Commun 373 (1): 99-103.Hendrickson SL, et al. (2010) Genetic variants in nuclear-encoded mitochondrial genes influence aids progression. PLoS One 5 (9): e12862.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65 kDa
NCBI Official Full Name
Homo sapiens HemK methyltransferase family member 1 (HEMK1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
HemK methyltransferase family member 1
NCBI Official Symbol
HEMK1
NCBI Official Synonym Symbols
HEMK; MTQ1
NCBI Protein Information
hemK methyltransferase family member 1
UniProt Protein Name
HemK methyltransferase family member 1
Protein Family
UniProt Gene Name
HEMK1
UniProt Synonym Gene Names
HEMK
Uniprot Description
HEMK1: N5-glutamine methyltransferase responsible for the methylation of the GGQ triplet of the mitochondrial translation release factor MTRF1L. Belongs to the protein N5-glutamine methyltransferase family.
Protein type: Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; EC 2.1.1.-; Lipid Metabolism - androgen and estrogen; Methyltransferase; Other Amino Acids Metabolism - selenoamino acid
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: mitochondrion
Molecular Function: protein binding; S-adenosylmethionine-dependent methyltransferase activity
Research Articles on HEMK1
Similar Products
Product Notes
The HEMK1 hemk1 (Catalog #AAA8102234) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HEMK1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HEMK1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-1:400. Researchers should empirically determine the suitability of the HEMK1 hemk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEMK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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