SDS-PAGE
TET3 recombinant protein
TET3 recombinant protein
SDS-PAGE
SDS-PAGE
Scientific Background: TET3 or Tet methylcytosine deoxygenase 3 is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. TET3 which is a member of the ten-eleven translocation (TET) gene family is involved in myelopoiesis, and defects in TET3 have been associated with several myeloproliferative disorders. TET plays an important role in embryonic stem cell maintenance and inner cell mass cell specification (1). TET3 play an important role in the DNA methylation process (2).
2. Langemeijer.et al : Acquired mutations in TET3 are common in myelodysplastic syndromes. Nature Genet. 41: 838-842, 2009.
NCBI and Uniprot Product Information
NCBI Description
Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]
Uniprot Description
Function: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin
By similarity. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Ref.6
Catalytic activity: DNA 5-methylcytosine + 2-oxoglutarate + O2 = DNA 5-hydroxymethylcytosine + succinate + CO2.
Cofactor: Binds 1 Fe2+ ion per subunit
By similarity.
Subunit structure: Interacts with HCFC1 and OGT. Ref.6 Ref.7
Subcellular location: Nucleus
By similarity. Cytoplasm
By similarity. Note: At the zygotic stage, localizes in the male pronucleus, while it localizes to the cytoplasm at other preimplantation stages
By similarity.
Tissue specificity: Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes. Ref.5
Developmental stage: Expressed in fetal brain but not adult brain. Ref.5
Sequence similarities: Belongs to the TET family.
Caution: Subsequent steps in cytosine demethylation are subject to discussion. According to a first model cytosine demethylation occurs through deamination of 5hmC into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. According to another model, cytosine demethylation is rather mediated via conversion of 5hmC into 5fC and 5caC, followed by excision by TDG.
Sequence caution: The sequence AAH22243.1 differs from that shown. Reason: Erroneous initiation. The sequence AAX93057.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAA23697.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on TET3
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Product Notes
The TET3 tet3 (Catalog #AAA515283) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant human TET3 (724-end) was expressed by baculovirus in Sf9 insect cells. AAA Biotech's TET3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TET3 tet3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TET3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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