Immunohistochemistry - Paraffin (IHC)
(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse anti-Human TET3 Monoclonal Antibody | anti-TET3 antibody
Anti-TET3 Antibody (aa241-568, clone 11E11) IHC-plus
WB (1:2000)
Immunohistochemistry - Paraffin (IHC)
(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry - Paraffin (IHC)
(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TET3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TET3.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TET3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TET3.)
NCBI and Uniprot Product Information
NCBI Description
Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]
Uniprot Description
TET3: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Conversion into 5hmC initiates a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. Belongs to the TET family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.n2; Oxidoreductase
Chromosomal Location of Human Ortholog: 2p13.1
Cellular Component: cytoplasm; female pronucleus; male pronucleus
Molecular Function: DNA binding; metal ion binding; protein binding
Biological Process: gene expression; positive regulation of transcription from RNA polymerase II promoter; protein amino acid O-linked glycosylation; regulation of gene expression, epigenetic
Research Articles on TET3
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Product Notes
The TET3 tet3 (Catalog #AAA249272) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-TET3 Antibody (aa241-568, clone 11E11) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TET3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P (1:15, 10 ug/ml) WB (1:2000). Researchers should empirically determine the suitability of the TET3 tet3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TET3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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