Galectin 9 Recombinant Antibody | Gal-9 recombinant antibody

Anti-Galectin 9 [RG9-35]

Cat. Num. AAA488482

• Gene Names: Lgals9; gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Functional Assay
• Purity: Protein A Affinity Purified
• Synonyms: Galectin 9; Monoclonal Recombinant Antibody; Anti-Galectin 9 [RG9-35]; ecalectin; Gal-9; galectin 9; Galectin-9; HUAT; lectin; galactose binding; soluble 5; LGALS9A; tumor antigen HOM-HD-21; urate transporter/channel protein.; Gal-9 recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Clone Number: RG9-35
• Specificity: This antibody is specific for murine galectin-9, a 40 kDa S-type lectin that is expressed by various cell types including lymphocytes, thymocytes, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, epithelial cells, and endothelial cells. Galectin-9 binds to beta-galactosides and can serve as a ligand for TIM-3 (CD366). The protein is implicated in both innate and adaptive immune responses specifically, induction of cytokine secretion by macrophages, bactericidal functions, promotion of dendritic cell maturation, regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 322

• Applicable Applications for Gal-9 recombinant antibody: Flow Cytometry (FC/FACS), Blocking (BL), Functional Assay (FA)
• Antibody Type: Recombinant Antibody
• Antibody Species: Mouse
• Original Species Name: Rat
• Immunogen: This antibody was raised by immunising rats with recombinant mouse galectin-9.
• Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Clone Note: When this antibody was first generated and characterised, it was shown to bind with galectin-9 but not with either galectin-1 or galectin-3, and to be capable of blocking Tim-3-Fc binding to galectin-9 and inhibiting galectin-9-induced Th1 cell death in vitro (Fukushima et al, 2008). This antibody has been used in FACS analyses, for instance, to describe a novel mechanism where Tim-3 binds HMGB-1 on virus-specific CD8+ Treg cells suppressing proliferation of CD8+ T effector cells during acute adenoviral infection (Dolina et al, 2014), and to indicate a crucial role for Tim-3 in phagocytosis of apoptotic cells and cross-presentation (Nakayama et al, 2009). In addition, this antibody has been used in vivo to study the roles of galectin-9 in the development of experimental allergic conjunctivitis in mice (Fukushima et al, 2008) and to demonstrate that constitutive expression of Gal-9 plays an immunosuppressive role in corneal allografts (Shimmura-Tomita et al, 2013).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Related Product Information for Gal-9 recombinant antibody

Recombinant monoclonal antibody to Galectin 9. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma RG9-35.

Product Categories/Family for Gal-9 recombinant antibody

Immunology; Cell Signalling; T Cells; Costimulation; Immuno-oncology

References

Fukushima et al. Roles of galectin-9 in the development of experimental allergic conjunctivitis in mice. Int Arch Allergy Immunol. 2008;146(1):36-43. PMID:18087160
Original Published Journal: Int Arch Allergy Immunol. 2008;146(1):36-43.
Publication Note: Describe the original generation of this antibody and its subsequent use in in vitro and in vivo functional assays to study the roles of galectin-9 in the development of experimental allergic conjunctivitis in mice.

NCBI and Uniprot Product Information

• NCBI GI #: 226531139
• NCBI GeneID: 16859
• NCBI Accession #: NP_001152773.1
• NCBI GenBank Nucleotide #: NP_001152773.1
• UniProt Accession #: O08573
• NCBI Official Full Name: galectin-9 isoform 2
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 9
• NCBI Official Symbol: Lgals9
• NCBI Official Synonym Symbols: gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
• NCBI Protein Information: galectin-9
• UniProt Protein Name: Galectin-9
• UniProt Gene Name: Lgals9
• UniProt Synonym Gene Names: Gal-9
• UniProt Entry Name: LEG9_MOUSE

Uniprot Description

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Cellular Component: cytoplasm; extracellular region

Molecular Function: galactoside binding; carbohydrate binding

Biological Process: heterophilic cell adhesion