Western Blot (WB)
(Western blot detection of RPA70 in Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
RPA70 Monoclonal Antibody | anti-RPA70 antibody
Anti-RPA70 Mouse mAb
Source: Human
ICC: 1:100
Other: 1:1000
Western Blot (WB)
(Western blot detection of RPA70 in Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Western Blot (WB)
(Western blot detection of RPA70 in Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysates using RPA70 mouse mAb.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysates using RPA70 mouse mAb.)
Western Blot (WB)
(Western blot detection of RPA70 in Hela, 293T, C6, 3T3 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Western Blot (WB)
(Western blot detection of RPA70 in Hela, 293T, C6, 3T3 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Western Blot (WB)
(Western blot detection of RPA70 in HUVEC, Hacat, 3T3, MCF7 and A549 cell lysates using RPA70 mouse mAb (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Western Blot (WB)
(Western blot detection of RPA70 in HUVEC, Hacat, 3T3, MCF7 and A549 cell lysates using RPA70 mouse mAb (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
NCBI and Uniprot Product Information
Uniprot Description
RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: DNA replication factor A complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body
Molecular Function: damaged DNA binding; protein binding; single-stranded DNA binding
Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA recombination; DNA repair; DNA replication; DNA-dependent DNA replication; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; mismatch repair; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; protein sumoylation; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair
Research Articles on RPA70
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Product Notes
The RPA70 rpa1 (Catalog #AAA475113) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPA70 Mouse mAb reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPA70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 1:1000 ICC: 1:100 Other: 1:1000. Researchers should empirically determine the suitability of the RPA70 rpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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