Mouse RPA70 Monoclonal Antibody | anti-RPA70 antibody

RPA70 Monoclonal Antibody

Cat. Num. AAA9400191

• Gene Names: RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: RPA70; Monoclonal Antibody; RPA70 Monoclonal Antibody; Dmrpa1; Drosophila Replication Protein A; DRPA; HSSB; Human single stranded DNA binding protein; MST075; MSTP075; p70; REPA1; Replication factor A; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; anti-RPA70 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 8C3-D12-H10
• Specificity: This antibody detects endogenous levels of RPA70 and does not cross-react with related proteins.
• Purity/Purification: Affinity purified
• Form/Format: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Sequence Length: 616

• Applicable Applications for anti-RPA70 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western blotting: 1:1000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Purified recombinant human RPA70 protein fragments expressed in E Coli.
• Target Name: RPA70
• Preparation and Storage: Store at -20 degree C

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using anti-RPA70 antibody (dilution 1:100).)

Western Blot (WB)

(Western blot detection of RPA70 in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)

Western Blot (WB)

(Western blot detection of RPA70 antibody in Hela,293T,C6,3T3 and K562 cell lysates using RPA70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)

Western Blot (WB)

(Western blot detection of RPA70 antibody in HUVEC,Hacat,3T3,MCF7 and A549 cell lysates using RPA70 antibody (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using RPA70 antibody.)

Related Product Information for anti-RPA70 antibody

Plays an essential role in several cellular processes in DNA metabolism including replication,recombination and DNA repair.Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Product Categories/Family for anti-RPA70 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506583
• NCBI GeneID: 6117
• NCBI Accession #: NP_002936.1
• NCBI GenBank Nucleotide #: NM_002945.3
• UniProt Accession #: P27694; Q59ES9; A8K0Y9
• Molecular Weight: 68,138 Da
• NCBI Official Full Name: replication protein A 70 kDa DNA-binding subunit
• NCBI Official Synonym Full Names: replication protein A1, 70kDa
• NCBI Official Symbol: RPA1
• NCBI Official Synonym Symbols: HSSB; RF-A; RP-A; REPA1; RPA70; MST075
• NCBI Protein Information: replication protein A 70 kDa DNA-binding subunit
• UniProt Protein Name: Replication protein A 70 kDa DNA-binding subunit
• UniProt Gene Name: RPA1
• UniProt Synonym Gene Names: REPA1; RPA70; RP-A p70; RF-A protein 1
• UniProt Entry Name: RFA1_HUMAN

Uniprot Description

RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: male germ cell nucleus; nucleoplasm; lateral element; PML body; DNA replication factor A complex; nucleus

Molecular Function: protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding

Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; homeostasis of number of cells within a tissue; nucleotide-excision repair; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; positive regulation of cell proliferation; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; hemopoiesis; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA-dependent DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle