Mouse anti-Human, Mouse RPA70 Monoclonal Antibody | anti-RPA1 antibody

RPA70 Antibody

Cat. Num. AAA9215454

• Gene Names: RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunoprecipitation
• Synonyms: RPA70; Monoclonal Antibody; RPA70 Antibody; Purified Mouse Monoclonal Antibody (Mab); Dmrpa1; Drosophila Replication Protein A; DRPA; DSSB; HSSB; Human single stranded DNA binding protein; MST075; MSTP075; p70; REPA 1; REPA1; Replication factor A; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; Replication protein A 70kDa DNA binding subunit; Replication protein A1 (70kD); Replication protein A1 70kDa; Replication protein A1; RF A; RF-A protein 1; RFA; RFA1_HUMAN; RP A; RP-A p70; RPA 1; RPA 70; RPA; RPA1; Single stranded binding protein 70; Single stranded DNA binding protein; Single-stranded DNA-binding protein; Ssb70.; anti-RPA1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Sequence Length: 616

• Applicable Applications for anti-RPA1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB ~~ 1:1000; ICC ~~ 1:100; IP ~~ 1:500
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of RPA70 in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using RPA70 mouse mAb.)

Related Product Information for anti-RPA1 antibody

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2- containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).

References

Erdile L.F.,et al.J. Biol. Chem. 266:12090-12098(1991). Erdile L.F.,et al.J. Biol. Chem. 268:2268-2268(1993). Ota T.,et al.Nat. Genet. 36:40-45(2004). Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases. Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 4506583
• NCBI GeneID: 6117
• NCBI Accession #: NP_002936.1
• NCBI GenBank Nucleotide #: NM_002945.3
• UniProt Accession #: P27694; Q59ES9; A8K0Y9
• Molecular Weight: 68138
• NCBI Official Full Name: replication protein A 70 kDa DNA-binding subunit
• NCBI Official Synonym Full Names: replication protein A1
• NCBI Official Symbol: RPA1
• NCBI Official Synonym Symbols: HSSB; RF-A; RP-A; REPA1; RPA70; MST075
• NCBI Protein Information: replication protein A 70 kDa DNA-binding subunit
• UniProt Protein Name: Replication protein A 70 kDa DNA-binding subunit
• Protein Family: Replication factor
• UniProt Gene Name: RPA1
• UniProt Synonym Gene Names: REPA1; RPA70; RP-A p70; RF-A protein 1
• UniProt Entry Name: RFA1_HUMAN

NCBI Description

RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: male germ cell nucleus; nucleoplasm; lateral element; PML body; DNA replication factor A complex; nuclear chromosome, telomeric region; nucleus

Molecular Function: protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding

Biological Process: meiosis; error-prone postreplication DNA repair; DNA strand elongation during DNA replication; DNA recombination; DNA recombinase assembly; base-excision repair; transcription-coupled nucleotide-excision repair; double-strand break repair; positive regulation of cell proliferation; telomere maintenance via recombination; hemopoiesis; nucleotide-excision repair, DNA damage removal; DNA replication; DNA-dependent DNA replication; telomere maintenance; mismatch repair; in utero embryonic development; DNA damage response, detection of DNA damage; DNA repair; post-translational protein modification; double-strand break repair via homologous recombination; telomere maintenance via semi-conservative replication; bypass DNA synthesis; cellular protein metabolic process; protein sumoylation; homeostasis of number of cells within a tissue; nucleotide-excision repair; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; G1/S transition of mitotic cell cycle

Uniprot Description

RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: DNA replication factor A complex; lateral element; male germ cell nucleus; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body

Molecular Function: chromatin binding; damaged DNA binding; metal ion binding; protein binding; single-stranded DNA binding

Biological Process: base-excision repair; bypass DNA synthesis; cellular protein metabolic process; DNA damage response, detection of DNA damage; DNA recombination; DNA repair; DNA replication; DNA strand elongation during DNA replication; DNA-dependent DNA replication; double-strand break repair; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; hemopoiesis; homeostasis of number of cells within a tissue; in utero embryonic development; meiosis; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; positive regulation of cell proliferation; post-translational protein modification; protein sumoylation; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair