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Western Blot (WB)
(Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.)
Rabbit anti-Human RPA70 Monoclonal Antibody | anti-RPA70 antibody
RPA70 Antibody
Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
RPA70; Monoclonal Antibody; RPA70 Antibody; Dmrpa1 antibody; Drosophila Replication Protein A antibody; DRPA antibody; HSSB antibody; Human single stranded DNA binding protein antibody; MST075 antibody; MSTP075 antibody; p70 antibody; REPA1 antibody; Replication factor A antibody; Replication factor A protein 1 antibody; Replication protein A 70 kDa DNA-binding subunit antibody; Replication protein A 70kDa DNA binding subunit antibody; Replication protein A1 70kDa antibody; Replication protein A1 antibody; RF A antibody; RF-A protein 1 antibody; RFA antibody; RFA1_HUMAN antibody; RP A antibody; RP-A p70 antibody; RPA 70 antibody; RPA antibody; rpa1 antibody; Single stranded binding protein 70 antibody; Single-stranded DNA-binding protein antibody; anti-RPA70 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JB75-32
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RPA70 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
ICC: 1:50-1:100
FC/FACS: 1:50-1:100
ICC: 1:50-1:100
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.)
Western Blot (WB)
(Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.)
Immunocytochemistry (ICC)
(ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-RPA70 antibody
The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.
Product Categories/Family for anti-RPA70 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
616
NCBI Official Full Name
replication protein A 70 kDa DNA-binding subunit
NCBI Official Synonym Full Names
replication protein A1, 70kDa
NCBI Official Symbol
RPA1
NCBI Official Synonym Symbols
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
NCBI Protein Information
replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; replication factor A protein 1; single-stranded DNA-binding protein
UniProt Protein Name
Replication protein A 70 kDa DNA-binding subunit
UniProt Gene Name
RPA1
UniProt Synonym Gene Names
REPA1; RPA70; RP-A p70
UniProt Entry Name
RFA1_HUMAN
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Product Notes
The RPA70 rpa1 (Catalog #AAA9436571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPA70 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPA70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:500 ICC: 1:50-1:100 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the RPA70 rpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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