Typical Testing Data/Standard Curve
Rat Utrophin ELISA Kit | UTRN elisa kit
Rat Utrophin ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||UTRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UTRN antibody and an UTRN-HRP conjugate. The assay sample and buffer are incubated together with UTRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UTRN concentration since UTRN from samples and UTRN-HRP conjugate compete for the anti-UTRN antibody binding site. Since the number of sites is limited, as more sites are occupied by UTRN from the sample, fewer sites are left to bind UTRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UTRN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene shares both structural and functional similarities with the dystrophin gene. It contains an actin-binding N-terminus, a triple coiled-coil repeat central region, and a C-terminus that consists of protein-protein interaction motifs which interact with dystroglycan protein components. The protein encoded by this gene is located at the neuromuscular synapse and myotendinous junctions, where it participates in post-synaptic membrane maintenance and acetylcholine receptor clustering. Mouse studies suggest that this gene may serve as a functional substitute for the dystrophin gene and therefore, may serve as a potential therapeutic alternative to muscular dystrophy which is caused by mutations in the dystrophin gene. Alternative splicing of the utrophin gene has been described; however, the full-length nature of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
utrophin: May play a role in anchoring the cytoskeleton to the plasma membrane.
Protein type: Motility/polarity/chemotaxis; Dystrophin complex
Chromosomal Location of Human Ortholog: 6q24
Cellular Component: dystrophin-associated glycoprotein complex; nucleoplasm; filopodium membrane; postsynaptic membrane; cytoskeleton; protein complex; membrane; cytoplasm; plasma membrane; cell junction; neuromuscular junction; filopodium
Molecular Function: integrin binding; protein binding; zinc ion binding; actin binding; protein kinase binding; vinculin binding
Biological Process: muscle development; muscle contraction; positive regulation of cell-matrix adhesion
Research Articles on UTRN
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Product Notes
The Rat UTRN utrn (Catalog #AAA750018) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA750018 ELISA Kit recognizes Rat UTRN. It is sometimes possible for the material contained within the vial of "Utrophin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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