Mouse Cereblon (CRBN) ELISA Kit | CRBN elisa kit

Mouse Cereblon (CRBN) ELISA Kit

Cat. Num. AAA454369

• Gene Names: Crbn; piL; AF229032; AW108261; 2610203G15Rik; 2900045O07Rik
• Reactivity: Mouse
• Synonyms: Cereblon (CRBN); Mouse Cereblon (CRBN) ELISA Kit; MRT2A; Mental Retardation; Non-Syndromic; Autosomal Recessive 2A; CRBN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CPS1. No significant cross-reactivity or interference between CPS1 and analogues was observed.
• Sequence Length: 444

• Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CPS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CPS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRBN elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CPS1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CPS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CPS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CPS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 90403612
• NCBI GeneID: 58799
• NCBI Accession #: NP_067424.2
• NCBI GenBank Nucleotide #: NM_021449.3
• UniProt Accession #: Q8C7D2; Q3TVC2; Q5RJV6; Q6IS49; Q80XJ1; Q8BP45; Q8C6B7; Q9JKR4
• Molecular Weight: 50,809 Da
• NCBI Official Full Name: protein cereblon isoform 1
• NCBI Official Synonym Full Names: cereblon
• NCBI Official Symbol: Crbn
• NCBI Official Synonym Symbols: piL; AF229032; AW108261; 2610203G15Rik; 2900045O07Rik
• NCBI Protein Information: protein cereblon
• UniProt Protein Name: Protein cereblon
• Protein Family: Protein cereblon
• UniProt Gene Name: Crbn
• UniProt Synonym Gene Names: Protein PiL

NCBI Description

This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic mental retardation. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (). May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1 ().