Human Protein cereblon ELISA Kit | CRBN elisa kit

Human Protein cereblon ELISA Kit

Cat. Num. AAA760148

• Gene Names: Crbn; piL; AF229032; AW108261; 2610203G15Rik; 2900045O07Rik
• Reactivity: Human
• Synonyms: Protein cereblon; Human Protein cereblon ELISA Kit; CRBN/Protein cereblon/DKFZp781K0715/MGC27358/MRT2A/AD-006; CRBN elisa kit

• Reactivity: Human
• Sequence Length: 444

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CRBN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CRBN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CRBN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CRBN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CRBN can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 90403612
• NCBI GeneID: 58799
• NCBI Accession #: NP_067424.2
• NCBI GenBank Nucleotide #: NM_021449.3
• Molecular Weight: 50,809 Da
• NCBI Official Full Name: protein cereblon isoform 1
• NCBI Official Synonym Full Names: cereblon
• NCBI Official Symbol: Crbn
• NCBI Official Synonym Symbols: piL; AF229032; AW108261; 2610203G15Rik; 2900045O07Rik
• NCBI Protein Information: protein cereblon
• UniProt Protein Name: Protein cereblon
• Protein Family: Protein cereblon
• UniProt Gene Name: Crbn
• UniProt Synonym Gene Names: Protein PiL
• UniProt Entry Name: CRBN_MOUSE

NCBI Description

This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic mental retardation. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

CRBN: Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A). MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs. Belongs to the CRBN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Cellular Component: cytoplasm; nucleolus; nucleus

Biological Process: negative regulation of protein homooligomerization; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination