Human Metallothionein 2 (MT2) ELISA Kit | MT2 elisa kit

Human Metallothionein 2 (MT2) ELISA Kit

Cat. Num. AAA2703825

• Gene Names: MT2A; MT2; MT-2; MT-II
• Reactivity: Human
• Synonyms: Metallothionein 2 (MT2); Human Metallothionein 2 (MT2) ELISA Kit; MT2A; CES1; MT2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Metallothionein 2 (MT2).; No significant cross-reactivity or interference between Metallothionein 2 (MT2) and analogues was observed.
• Sequence Length: 61

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Metallothionein 2 (MT2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Metallothionein 2 (MT2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MT2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metallothionein 2 (MT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metallothionein 2 (MT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metallothionein 2 (MT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Metallothionein 2 (MT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1495466
• NCBI GeneID: 4502
• UniProt Accession #: P02795; Q14823; Q2HXR9; Q53XT9
• Molecular Weight: 6,042 Da
• NCBI Official Full Name: Metallothionein 2
• NCBI Official Synonym Full Names: metallothionein 2A
• NCBI Official Symbol: MT2A
• NCBI Official Synonym Symbols: MT2; MT-2; MT-II
• NCBI Protein Information: metallothionein-2
• UniProt Protein Name: Metallothionein-2
• Protein Family: Metallothionein
• UniProt Gene Name: MT2A
• UniProt Synonym Gene Names: CES1; MT2; MT-2; MT-II

NCBI Description

This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions, altering the intracellular concentration of heavy metals in the cell. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. The encoded protein interacts with the protein encoded by the homeobox containing 1 gene in some cell types, controlling intracellular zinc levels, affecting apoptotic and autophagy pathways. Some polymorphisms in this gene are associated with an increased risk of cancer. [provided by RefSeq, Sep 2017]

Uniprot Description

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.