Typical Testing Data/Standard Curve (for reference only)
Mouse Uroporphyrinogen Decarboxylase (UROD) ELISA Kit | UROD elisa kit
Mouse Uroporphyrinogen Decarboxylase (UROD) ELISA Kit
Gene Names
Urod; UPD; Uro-d; AI323803
Reactivity
Mouse
Synonyms
Uroporphyrinogen Decarboxylase (UROD); Mouse Uroporphyrinogen Decarboxylase (UROD) ELISA Kit; PCT; Uroporphyrinogen III Decarboxylase; UROD elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of UROD. No significant cross-reactivity or interference between UROD and analogues was observed.
Sequence Length
367
Samples
Tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
<0.056ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UROD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UROD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for UROD elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of UROD in mouse tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to UROD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to UROD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UROD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of UROD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to UROD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to UROD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UROD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of UROD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,692 Da
NCBI Official Full Name
uroporphyrinogen decarboxylase
NCBI Official Synonym Full Names
uroporphyrinogen decarboxylase
NCBI Official Symbol
Urod
NCBI Official Synonym Symbols
UPD; Uro-d; AI323803
NCBI Protein Information
uroporphyrinogen decarboxylase
UniProt Protein Name
Uroporphyrinogen decarboxylase
UniProt Gene Name
Urod
UniProt Synonym Gene Names
UPD; URO-D
NCBI Description
This gene encodes an enzyme that catalyzes the conversion of uroporphyrinogen-III to coproporphyrinogen-III, an intermediate step in heme biosynthesis. Homozygous knockout mice for this gene exhibit embryonic lethality. [provided by RefSeq, Aug 2015]
Uniprot Description
Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.
Research Articles on UROD
1. accelerated development of uroporphyria in mice heterozygous for deletion at uroporphyrinogen locus
Similar Products
Product Notes
The Mouse UROD urod (Catalog #AAA457231) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457231 ELISA Kit recognizes Mouse UROD. It is sometimes possible for the material contained within the vial of "Uroporphyrinogen Decarboxylase (UROD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
