Human Cereblon (CRBN) RTU ELISA Kit | CRBN rtu elisa kit

Human Cereblon (CRBN) ELISA Kit

Cat. Num. AAA458556

• Gene Names: CRBN; MRT2; MRT2A
• Reactivity: Human
• Synonyms: Cereblon (CRBN); Human Cereblon (CRBN) ELISA Kit; MRT2A; Mental Retardation; Non-Syndromic; Autosomal Recessive 2A; CRBN rtu elisa kit

• Reactivity: Human
• Sequence Length: 441

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.125ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRBN rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRBN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CRBN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRBN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CRBN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 291045198
• NCBI GeneID: 51185
• NCBI Accession #: NP_001166953.1
• NCBI GenBank Nucleotide #: NM_001173482.1
• UniProt Accession #: Q96SW2; Q6AI62; Q6NVZ0; Q9UHW4; B2R6H4; C9IZA9; C9JAH6
• Molecular Weight: 50,475 Da
• NCBI Official Full Name: protein cereblon isoform 2
• NCBI Official Synonym Full Names: cereblon
• NCBI Official Symbol: CRBN
• NCBI Official Synonym Symbols: MRT2; MRT2A
• NCBI Protein Information: protein cereblon
• UniProt Protein Name: Protein cereblon
• Protein Family: Protein cereblon
• UniProt Gene Name: CRBN

NCBI Description

This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.