Western Blot (WB)
((1ug/ml) staining of NSO lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RHBG Polyclonal Antibody | anti-RHBG antibody
Goat anti-RHBG (mouse) Antibody
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RHBG; Polyclonal Antibody; Goat anti-RHBG (mouse) Antibody; Goat Anti-RHBG Antibody; Rh family; B glycoprotein (gene/pseudogene); SLC42A2; Rh type B glycoprotein; Rhesus blood group; B glycoprotein; B glycoprotein (gene/pseudogene) antibody; Rh type B glycoprotein antibody; B glycoprotein antibody; SLC42A2 antibody; RHBG antibody; RHBG (mouse); anti-RHBG antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PLRGGESDTR
Sequence Length
455
Applicable Applications for anti-RHBG antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 55kDa band observed in Mouse Kidney lysates and in lysates of cell lines NIH3T3 and NSO (calculated MW of 49.5kDa according to NP_067350.2). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 55kDa band observed in Mouse Kidney lysates and in lysates of cell lines NIH3T3 and NSO (calculated MW of 49.5kDa according to NP_067350.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-PLRGGESDTR, from the C Terminus of the protein sequence according to NP_067350.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of NSO lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of NSO lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,543 Da
NCBI Official Full Name
ammonium transporter Rh type B
NCBI Official Synonym Full Names
Rhesus blood group-associated B glycoprotein
NCBI Official Symbol
Rhbg
NCBI Protein Information
ammonium transporter Rh type B
UniProt Protein Name
Ammonium transporter Rh type B
Protein Family
UniProt Gene Name
Rhbg
UniProt Synonym Gene Names
Rh family type B glycoprotein; Rh type B glycoprotein
UniProt Entry Name
RHBG_MOUSE
Uniprot Description
RHBG: Functions as a specific ammonium transporter. Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family
Cellular Component: anchored to plasma membrane; basolateral plasma membrane; cytoplasmic vesicle; integral to membrane; integral to plasma membrane; membrane; plasma membrane
Molecular Function: ammonium transmembrane transporter activity; ankyrin binding
Biological Process: ammonium transport; nitrogen utilization; organic cation transport; transport
Research Articles on RHBG
Similar Products
Product Notes
The RHBG rhbg (Catalog #AAA422450) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RHBG (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RHBG can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 55kDa band observed in Mouse Kidney lysates and in lysates of cell lines NIH3T3 and NSO (calculated MW of 49.5kDa according to NP_067350.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the RHBG rhbg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PLRGGESDTR. It is sometimes possible for the material contained within the vial of "RHBG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
