Western Blot (WB)
(AQP12B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the AQP12B antibody detected the AQP12B protein (arrow).)
Rabbit anti-Human AQP12B Polyclonal Antibody | anti-AQP12B antibody
AQP12B, CT (AQP12B, Aquaporin-12B)
Gene Names
AQP12B; INSSA3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
AQP12B; Polyclonal Antibody; CT (AQP12B; Aquaporin-12B); Anti -AQP12B; anti-AQP12B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-AQP12B antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunogen
AQP12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the C-terminal region of human AQP12B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(AQP12B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the AQP12B antibody detected the AQP12B protein (arrow).)
Western Blot (WB)
(AQP12B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the AQP12B antibody detected the AQP12B protein (arrow).)
Immunohistochemistry (IHC)
(AQP12B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AQP12B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(AQP12B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AQP12B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(AQP12B Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(AQP12B Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-AQP12B antibody
Aquaporins facilitate the transport of water and small neutral solutes across cell membranes (By similarity).
Product Categories/Family for anti-AQP12B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,475 Da
NCBI Official Full Name
aquaporin-12B
NCBI Official Synonym Full Names
aquaporin 12B
NCBI Official Symbol
AQP12B
NCBI Official Synonym Symbols
INSSA3
NCBI Protein Information
aquaporin-12B; AQP-12B; insulin synthesis associated 3
UniProt Protein Name
Aquaporin-12B
Protein Family
UniProt Gene Name
AQP12B
UniProt Synonym Gene Names
AQP-12B
UniProt Entry Name
AQ12B_HUMAN
Uniprot Description
AQP12B: Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. Belongs to the MIP/aquaporin (TC 1.A.8) family. AQP11/AQP12 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter, aquaporin family; Transporter; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: integral to membrane
Molecular Function: transporter activity
Biological Process: transport
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Product Notes
The AQP12B aqp12b (Catalog #AAA6003751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AQP12B, CT (AQP12B, Aquaporin-12B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AQP12B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the AQP12B aqp12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AQP12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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