Western Blot (WB)
((1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat IRAK4 Polyclonal Antibody | anti-IRAK4 antibody
Goat anti-IRAK4 (N Terminus) Antibody
Western Blot: Approx 50kDa band observed in lysates of cell lines A431, HeLa and NIH3T3 (calculated MW of 51.5kDa according to NP_057207.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; TKL group; IRAK family
Chromosomal Location of Human Ortholog: 12q12
Cellular Component: cytoplasm; cytosol; endosome membrane; extracellular space; nucleus; plasma membrane
Molecular Function: ATP binding; interleukin-1 receptor binding; magnesium ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: cytokine and chemokine mediated signaling pathway; cytokine production; innate immune response; JNK cascade; MyD88-dependent toll-like receptor signaling pathway; neutrophil mediated immunity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of smooth muscle cell proliferation; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway
Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency
Research Articles on IRAK4
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Product Notes
The IRAK4 irak4 (Catalog #AAA420864) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IRAK4 (N Terminus) Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRAK4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 50kDa band observed in lysates of cell lines A431, HeLa and NIH3T3 (calculated MW of 51.5kDa according to NP_057207.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the IRAK4 irak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NKPITPSTYV RC. It is sometimes possible for the material contained within the vial of "IRAK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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