Mouse anti-Human PAX-8 Monoclonal Antibody | anti-PAX-8 antibody

PAX-8

Cat. Num. AAA344007

• Reactivity: Human
• Synonyms: PAX-8; Monoclonal Antibody; PAX8; anti-PAX-8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 60

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Ovarian Carcinoma (Non-Mucinous Carcinoma), Thyroid Carcinoma, Renal Cell Carcinoma
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(PAX-8 [IHC008] on Thyroid Gland)

Related Product Information for anti-PAX-8 antibody

Intended Use: The PAX8 [IHC008] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: PAX-8 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. This protein plays a role in development of thyroid follicular cells and the expression of thyroid-specific genes, with mutations in the PAX-8 gene linked to thyroid follicular carcinomas, atypical thyroid adenomas, and thyroid dysgenesis. The PAX-8 protein is expressed in simple ovarian inclusion cysts and non-ciliated mucosal cells of the fallopian tubes, but is absent from normal ovarian surface epithelial cells. PAX-8 is also not expressed in normal lung or lung carcinomas. Reports have associated PAX-8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX-8 as a useful marker for renal epithelial tumors, ovarian cancer, and for differential identifys in lung and neck tumors. Anti-PAX-8 can be useful in determining the primary site of invasive micropapillary carcinomas of ovary from bladder, lung, and breast, when used in adjunct with a panel of organ-specific markers such as uroplakin, mammaglobin, and TTF-1.

References

1. Tong GX, et al. Mod Pathol. 2009; 22:1218-27.
2. Nonaka D, et al. Mod Pathol. 2008; 21:192-200.
3. Tamara L, et al. Am J Surg Pathol. 2009; 33:1037-41.
4. Nikiforova MN, et al. Am J Surg Pathol. 2002; 26:1016-23.
5. Nonaka D, et al. Am J Surg Pathol. 2008; 32:1566-71.
6. Marques AR, et al. J Clin Endocrinol Metab. 2002; 87:3947-52.
7. Tina DP, et al. J Biol Chem. 2003; 278:3395-3402.
8. Bowen NJ, et al. Gynecol Oncol. 2007; 104:331-7.
9. Zhang P, et al. Pathol Int. 2006; 56:240-245.

NCBI and Uniprot Product Information

• NCBI GI #: 1850829
• NCBI GeneID: 7849
• UniProt Accession #: Q99573; Q06710
• Molecular Weight: 6,524 Da
• NCBI Official Full Name: Pax-8, partial
• NCBI Official Synonym Full Names: paired box 8
• NCBI Official Symbol: PAX8
• NCBI Protein Information: paired box protein Pax-8
• UniProt Protein Name: Pax-8 protein
• UniProt Gene Name: Pax-8

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]