Mouse Follicle-stimulating hormone ELISA Kit | FSH elisa kit

Mouse Follicle-stimulating hormone ELISA Kit

Cat. Num. AAA763205

• Gene Names: FSHR; LGR1; ODG1; FSHR1; FSHRO
• Reactivity: Mouse
• Synonyms: Follicle-stimulating hormone; Mouse Follicle-stimulating hormone ELISA Kit; FSH (Follicle-Stimulating Hormone); FSH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FSH. No significant cross-reactivity or interference between FSH and analogues was observed.
• Sequence Length: 669

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 2.344-150mIU/ml
• Sensitivity: 1.406mIU/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FSH elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 291575177
• NCBI GeneID: 2492
• NCBI Accession #: NP_852111.2
• NCBI GenBank Nucleotide #: NM_181446.2
• Molecular Weight: 81,974 Da
• NCBI Official Full Name: follicle-stimulating hormone receptor isoform 2
• NCBI Official Synonym Full Names: follicle stimulating hormone receptor
• NCBI Official Symbol: FSHR
• NCBI Official Synonym Symbols: LGR1; ODG1; FSHR1; FSHRO
• NCBI Protein Information: follicle-stimulating hormone receptor
• UniProt Protein Name: Follicle-stimulating hormone receptor
• Protein Family: Follicle-stimulating hormone receptor
• UniProt Gene Name: FSHR
• UniProt Synonym Gene Names: LGR1; FSH-R
• UniProt Entry Name: FSHR_HUMAN

NCBI Description

The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

FSHR: Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Defects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1); also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS). OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p21-p16

Cellular Component: integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: follicle-stimulating hormone receptor activity; peptide receptor activity, G-protein coupled; protein binding

Biological Process: adenylate cyclase activation; female gamete generation; female gonad development; follicle-stimulating hormone signaling pathway; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; gonad development; hormone-mediated signaling; male gonad development; positive regulation of phosphoinositide 3-kinase cascade; primary ovarian follicle growth; regulation of osteoclast differentiation; spermatogenesis

Disease: Ovarian Dysgenesis 1; Ovarian Hyperstimulation Syndrome; Twinning, Dizygotic