Rabbit PRDX6 Polyclonal Antibody | anti-PRDX6 antibody

PRDX6 Polyclonal Antibody

Cat. Num. AAA3013288

• Gene Names: PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence
• Purity: ProA affinity purified
• Synonyms: PRDX6; Polyclonal Antibody; PRDX6 Polyclonal Antibody; 1-Cys PRX; aiPLA2; NSGPx; 1 Cys; 1 Cys peroxiredoxin; 1 Cys PRX; 1 cysPrx; 1-Cys peroxiredoxin; 24 kDa protein; 9430088D19Rik; AA690119; Acidic calcium independent phospholipase A2; Acidic calcium-independent phospholipase A2; Antioxidant protein 2; AOP2; Aop2 rs3; Brp 12; Ciliary body glutathione peroxidase; CP 3; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1.; Epididymis secretory sperm binding protein Li 128m; GPx; HEL S 128m; KIAA0106; Liver 2D page spot 40; Ltw4; Lvtw 4; MGC46173; mKIAA0106; Non selenium glutathione peroxidase; Non-selenium glutathione peroxidase; ORF06; OTTHUMP00000032693; p29; Peroxiredoxin-6; Peroxiredoxin6; PHGPx; Phospholipase A2 lysosomal; PLA2; PRDX 6; Prdx5; Prdx6 rs3; PRX; Red blood cells page spot 12; Thiol specific antioxidant protein; anti-PRDX6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PRDX6 polyclonal antibody detects endogenous levels of PRDX6 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 224

• Applicable Applications for anti-PRDX6 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200; IP: 1:10-1:50
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of PRDX6 on Jurkat cells lysates using anti-PRDX6 antibody at 1/500 dilution. Positive control: Line 1: Hela Line 2:HepG2)

Related Product Information for anti-PRDX6 antibody

The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis. The human PRX IV gene is abundantly expressed in many tissues. PRX IV exists as a precursor protein, which is only detected in testis, and a processed secreted form. PRX V also exists as two forms, designated long and short. Like PRX IV, the long form of PRX V is highly expressed in testis. The short form of PRX V is more widely expressed, with high expression in liver, kidney, heart and lung. PRX VI, a 1-Cys peroxiredoxin (also known as antioxidant protein 2 or AOP2), is highly expressed in most tissues, particularly in epithelial cells. Localized to the cell cytosol, PRX VI functions independently of other peroxiredoxins and antioxidant proteins, specializing in antioxidant defense, lung phospholipid metabolism and protection of keratinocytes from cell death induced by reactive oxygen species.

NCBI and Uniprot Product Information

• NCBI GI #: 1718024
• NCBI GeneID: 9588
• UniProt Accession #: P30041; O08709; O35244
• Molecular Weight: 25kDa
• NCBI Official Full Name: Peroxiredoxin-6
• NCBI Official Synonym Full Names: peroxiredoxin 6
• NCBI Official Symbol: PRDX6
• NCBI Official Synonym Symbols: PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
• NCBI Protein Information: peroxiredoxin-6
• UniProt Protein Name: Peroxiredoxin-6
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX6
• UniProt Synonym Gene Names: AOP2; KIAA0106; 1-Cys PRX; aiPLA2; NSGPx
• UniProt Entry Name: PRDX6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDX6: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Belongs to the AhpC/TSA family. Rehydrin subfamily.

Protein type: Amino Acid Metabolism - phenylalanine; Oxidoreductase; Energy Metabolism - methane; EC 1.11.1.9; EC 1.11.1.15; Phospholipase

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: extracellular space; membrane; lysosome; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol

Molecular Function: antioxidant activity; phospholipase A2 activity; protein binding; peroxiredoxin activity; glutathione peroxidase activity

Biological Process: response to reactive oxygen species; phospholipid catabolic process; hydrogen peroxide catabolic process; response to oxidative stress