Mouse anti-Human MTAP Antibody | anti-MTAP antibody

MTAP (Tumor Suppressor Marker)

Cat. Num. AAA4381100

• Gene Names: MTAP; BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: MTAP; MTAP (Tumor Suppressor Marker); BDMF; DMSFH; DMSMFH; Epididymis luminal protein 249; HEL249; LGMBF; MeSAdo phosphorylase; Methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAPase; S-methyl-5''-thioadenosine phosphorylase; anti-MTAP antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rMTAP/1813
• Specificity: Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 283

• Applicable Applications for anti-MTAP antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HepG2, A431, HeLa or MCF-7 cells. Human kidney tissues (IHC).
• Immunogen: Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 9p21.3
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).)

SDS-Page

(SDS-PAGE Analysis Purified MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813). Confirmation of Purity and Integrity of Antibody.)

References

Nobori, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93: 6203-6208.

NCBI and Uniprot Product Information

• NCBI GI #: 47132622
• NCBI GeneID: 4507
• NCBI Accession #: NP_002442
• NCBI GenBank Nucleotide #: NP_002442.2
• UniProt Accession #: Q13126
• Molecular Weight: 31kDa
• NCBI Official Full Name: S-methyl-5'-thioadenosine phosphorylase
• NCBI Official Synonym Full Names: methylthioadenosine phosphorylase
• NCBI Official Symbol: MTAP
• NCBI Official Synonym Symbols: BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249
• NCBI Protein Information: S-methyl-5'-thioadenosine phosphorylase
• UniProt Protein Name: S-methyl-5'-thioadenosine phosphorylase
• UniProt Gene Name: MTAP
• UniProt Entry Name: MTAP_HUMAN

NCBI Description

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]