Rabbit anti-Human NLRP12 Polyclonal Antibody | anti-NLRP12 antibody

NLRP12, NT (NLRP12, NALP12, PYPAF7, RNO, NACHT, LRR and PYD domains-containing protein 12, Monarch-1, PYRIN-containing APAF1-like protein 7, Regulated by nitric oxide) (Azide free) (HRP)

Cat. Num. AAA6325767

• Gene Names: NLRP12; RNO; PAN6; RNO2; FCAS2; NALP12; PYPAF7; CLR19.3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NLRP12; Polyclonal Antibody; NT (NLRP12; NALP12; PYPAF7; RNO; NACHT; LRR and PYD domains-containing protein 12; Monarch-1; PYRIN-containing APAF1-like protein 7; Regulated by nitric oxide) (Azide free) (HRP); Regulated by nitric oxide; anti-NLRP12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NLRP12 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NLRP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-212 amino acids from the N-terminal region of human NLRP12.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NLRP12 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NLRP12 antibody detected the NLRP12 protein (arrow).)

Immunohistochemistry (IHC)

(NLRP12 Antibody (N-term) (AP14014a)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NLRP12 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NLRP12 antibody

This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Alternatively spliced transcript variants encoding distinct isoforms have been described but the full-length nature of some of these has not been determined.

Product Categories/Family for anti-NLRP12 antibody

Antibodies; Nitric Oxide

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Cummings, J.R., et al. Tissue Antigens 76(1):48-56(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Fahy, R.J., et al. Am. J. Respir. Crit. Care Med. 177(9):983-988(2008). Jeru, I., et al. Proc. Natl. Acad. Sci. U.S.A. 105(5):1614-1619(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 21955154
• NCBI GeneID: 91662
• NCBI Accession #: NP_653288.1; NP_150639.1
• NCBI GenBank Nucleotide #: NM_144687.2
• UniProt Accession #: P59046; Q8NEU4; Q9BY26
• Molecular Weight: 120,173 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 12 isoform 2
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 12
• NCBI Official Symbol: NLRP12
• NCBI Official Synonym Symbols: RNO; PAN6; RNO2; FCAS2; NALP12; PYPAF7; CLR19.3
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 12; monarch 1; monarch-1; OTTHUMP00000067226; OTTHUMP00000067227; OTTHUMP00000067228; OTTHUMP00000067229; regulated by nitric oxide; PYRIN-containing APAF1-like protein 7; NACHT, LRR and PYD containing protein
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 12
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP12
• UniProt Synonym Gene Names: NALP12; PYPAF7; RNO
• UniProt Entry Name: NAL12_HUMAN

Uniprot Description

NLRP12: May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK. Defects in NLRP12 are the cause of familial cold autoinflammatory syndrome type 2 (FCAS2). FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; caspase activator activity; ATP binding

Biological Process: release of cytoplasmic sequestered NF-kappaB; caspase activation; negative regulation of cytokine secretion; negative regulation of interleukin-6 biosynthetic process; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion; signal transduction; regulation of caspase activity; negative regulation of signal transduction; negative regulation of Toll signaling pathway; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; negative regulation of protein amino acid autophosphorylation; positive regulation of MHC class I biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 secretion; regulation of interleukin-18 biosynthetic process; positive regulation of inflammatory response

Disease: Familial Cold Autoinflammatory Syndrome 2