Decay Accelerating Factor (DAF) Recombinant Protein | DAF recombinant protein

Recombinant Decay Accelerating Factor (DAF)

Cat. Num. AAA2011036

• Gene Names: Cd55; Daf; Daf1; Daf-GPI; GPI-DAF
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Decay Accelerating Factor (DAF); Recombinant Decay Accelerating Factor (DAF); DAF recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- PILG RHSKFAEQSK VAYSCNNGFK QVPDKSNIVV CLENGQWSSH ETFCEKSCVA PERLSFASLK KEYLNMNFFP VGTIVEYECR PGFRKQPPLP GKATCLEDLV WSPVAQFCKK KSCPNPKDLD NGHINIPTGI LFGSEINFSC NPGYRLVGVS STFCSVTGNT VDWDDEFPVC TEIHCPEPPK INNGIMRGES DSYTYSQVVT YSCDKGFILV GNASIYCTVS KSDVGQWSSP PPRCIE
• Sequence Length: 390

• Applicable Applications for DAF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Pro47~Glu286 (Accession # Q61475) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 114326522
• NCBI GeneID: 13136
• NCBI Accession #: NP_034146.2
• NCBI GenBank Nucleotide #: NM_010016.2
• UniProt Accession #: Q61475; P97732; Q3TU32; Q4FJS4; Q61397; Q76N72; Q921P0; Q9R1C1
• Molecular Weight: 28.1kDa
• NCBI Official Full Name: complement decay-accelerating factor, GPI-anchored
• NCBI Official Synonym Full Names: CD55 antigen
• NCBI Official Symbol: Cd55
• NCBI Official Synonym Symbols: Daf; Daf1; Daf-GPI; GPI-DAF
• NCBI Protein Information: complement decay-accelerating factor, GPI-anchored; Cromer blood group; GPI anchor addition signal; decay accelerating factor 1; complement-glycosylphosphatidylinositol
• UniProt Protein Name: Complement decay-accelerating factor, GPI-anchored
• UniProt Gene Name: Cd55
• UniProt Synonym Gene Names: Cd55a; Daf; Daf1; DAF-GPI
• UniProt Entry Name: DAF1_MOUSE

Uniprot Description

CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, GPI anchor

Cellular Component: cell surface; membrane; apical plasma membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: viral receptor activity; enzyme inhibitor activity; lipid binding

Biological Process: immune system process; response to virus; negative regulation of catalytic activity; regulation of complement activation, classical pathway; innate immune response; complement activation, classical pathway