Rabbit anti-Human IMPDH1 Polyclonal Antibody | anti-IMPDH1 antibody

Rabbit Polyclonal to Human IMPDH1

Cat. Num. AAA248553

• Gene Names: IMPDH1; IMPD; RP10; IMPD1; LCA11; sWSS2608
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: IMPDH1; Polyclonal Antibody; Rabbit Polyclonal to Human IMPDH1; Anti-IMPDH1 Antibody (aa84-113) IHC-plus; IMP dehydrogenase 1; IMPD 1; IMPDH 1; IMPD1; LCA11; IMPD; IMPDH-I; SWSS2608; RP10; Human IMPDH1; anti-IMPDH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human IMPDH1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 599

• Applicable Applications for anti-IMPDH1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1:100), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH-conjugated synthetic peptide from N-terminal region of human IMPDH1.
• Immunogen Type: Synthetic peptide
• Immunogen: IMPDH1 antibody was raised against kLH-conjugated synthetic peptide from N-terminal region of human IMPDH1.
• Antigen Modification: aa84-113
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-IMPDH1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-IMPDH1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(IMPDH1 Antibody (N-term) Western blot of HL-60 cell line lysates (35 ug/lane). This demonstrates the IMPDH1 antibody detected the IMPDH1 protein (arrow).)

NCBI and Uniprot Product Information

• NCBI GI #: 34328930
• NCBI GeneID: 3614
• NCBI Accession #: NP_000874.2
• NCBI GenBank Nucleotide #: NM_000883.3
• UniProt Accession #: P20839; Q8N194; Q96NU2; A4D0Z6; A4D0Z7; A6NDW5; A6NNI6; B3KNP7; B3KVM8; B4DE09; C9JV30; J3KNX8
• Molecular Weight: 60,898 Da
• NCBI Official Full Name: inosine-5'-monophosphate dehydrogenase 1 isoform a
• NCBI Official Synonym Full Names: IMP (inosine 5'-monophosphate) dehydrogenase 1
• NCBI Official Symbol: IMPDH1
• NCBI Official Synonym Symbols: IMPD; RP10; IMPD1; LCA11; sWSS2608
• NCBI Protein Information: inosine-5'-monophosphate dehydrogenase 1; IMPD 1; IMPDH1; IMPDH 1; IMPDH-I; IMP dehydrogenase 1; IMP (inosine monophosphate) dehydrogenase 1
• UniProt Protein Name: Inosine-5'-monophosphate dehydrogenase 1
• Protein Family: Inosine-5'-monophosphate dehydrogenase
• UniProt Gene Name: IMPDH1
• UniProt Entry Name: IMDH1_HUMAN

NCBI Description

The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

IMPDH1: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant. Defects in IMPDH1 are the cause of Leber congenital amaurosis type 11 (LCA11). LCA11 is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the IMPDH/GMPR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.205; Xenobiotic Metabolism - drug metabolism - other enzymes; Oxidoreductase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 7q31.3-q32

Cellular Component: nucleoplasm; cytoplasm; nucleus; cell junction; cytosol

Molecular Function: DNA binding; nucleic acid binding; RNA binding; metal ion binding; nucleotide binding; IMP dehydrogenase activity

Biological Process: purine ribonucleoside monophosphate biosynthetic process; lymphocyte proliferation; nucleobase, nucleoside and nucleotide metabolic process; GMP biosynthetic process; purine base metabolic process

Disease: Leber Congenital Amaurosis 11; Retinitis Pigmentosa 10