Western Blot (WB)
(Western Blot: Sample: Mouse Heart Tissue.)
Rabbit anti-Mouse Decay Accelerating Factor (DAF) Polyclonal Antibody | anti-DAF antibody
Polyclonal Antibody to Decay Accelerating Factor (DAF)
MGHHHHHHSGSEF- PILG RHSKFAEQSK VAYSCNNGFK QVPDKSNIVV CLENGQWSSH ETFCEKSCVA PERLSFASLK KEYLNMNFFP VGTIVEYECR PGFRKQPPLP GKATCLEDLV WSPVAQFCKK KSCPNPKDLD NGHINIPTGI LFGSEINFSC NPGYRLVGVS STFCSVTGNT VDWDDEFPVC TEIHCPEPPK INNGIMRGES DSYTYSQVVT YSCDKGFILV GNASIYCTVS KSDVGQWSSP PPRCIE
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Store at 4oC for frequent use.
Aliquot and store at -20oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Mouse Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Mouse Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DAF, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DAF, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1. [provided by RefSeq, Nov 2015]
Uniprot Description
CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 1 E4|1 56.89 cM
Cellular Component: apical plasma membrane; cell surface; external side of plasma membrane; membrane; membrane raft; plasma membrane
Molecular Function: enzyme inhibitor activity; lipid binding; viral receptor activity
Biological Process: complement activation, classical pathway; immune system process; innate immune response; regulation of complement activation, classical pathway
Research Articles on DAF
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Product Notes
The DAF cd55 (Catalog #AAA2004075) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Decay Accelerating Factor (DAF) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Decay Accelerating Factor (DAF) can be used in a range of immunoassay formats including, but not limited to, WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the DAF cd55 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- PILG RHSKFAEQSK VAYSCNNGFK QVPDKSNIVV CLENGQWSSH ETFCEKSCVA PERLSFASLK KEYLNMNFFP VGTIVEYECR PGFRKQPPLP GKATCLEDLV WSPVAQFCKK KSCPNPKDLD NGHINIPTGI LFGSEINFSC NPGYRLVGVS STFCSVTGNT VDWDDEFPVC TEIHCPEPPK INNGIMRGES DSYTYSQVVT YSCDKGFILV GNASIYCTVS KSDVGQWSSP PPRCIE. It is sometimes possible for the material contained within the vial of "Decay Accelerating Factor (DAF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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