CD55/DAF Recombinant Protein | CD55 recombinant protein

CD55/DAF Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122765

• Gene Names: Cd55; Daf; Daf1; Daf-GPI; GPI-DAF
• Purity: >92% as determined by SDS-PAGE
• Synonyms: CD55/DAF; CD55/DAF Protein; Mouse; Recombinant (His Tag); Mouse CD55/DAF Protein (His Tag); Daf Protein; Daf-GPI Protein; Daf1 Protein; GPI-DAF Protein; CD55 molecule; decay accelerating factor for complement (Cromer blood group); CD55 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr361

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 35
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of mouse CD55 (Q61475)(Met 1-Thr 361) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CD55 recombinant protein

Background: CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.

Product Categories/Family for CD55 recombinant protein

Complement Activation Pathways

References

Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.

NCBI and Uniprot Product Information

• NCBI GI #: 114326522
• NCBI GeneID: 13136
• NCBI Accession #: NP_034146.2
• NCBI GenBank Nucleotide #: NM_010016.3
• UniProt Accession #: Q61475; P97732; Q3TU32; Q4FJS4; Q61397; Q76N72; Q921P0; Q9R1C1
• Molecular Weight: 42,618 Da
• NCBI Official Full Name: complement decay-accelerating factor, GPI-anchored preproprotein
• NCBI Official Synonym Full Names: CD55 molecule, decay accelerating factor for complement
• NCBI Official Symbol: Cd55
• NCBI Official Synonym Symbols: Daf; Daf1; Daf-GPI; GPI-DAF
• NCBI Protein Information: complement decay-accelerating factor, GPI-anchored
• UniProt Protein Name: Complement decay-accelerating factor, GPI-anchored
• Protein Family: Complement decay-accelerating factor
• UniProt Gene Name: Cd55
• UniProt Synonym Gene Names: Cd55a; Daf; Daf1; DAF-GPI

NCBI Description

This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1. [provided by RefSeq, Nov 2015]

Uniprot Description

CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1 E4|1 56.89 cM

Cellular Component: apical plasma membrane; cell surface; external side of plasma membrane; lipid raft; plasma membrane

Molecular Function: enzyme inhibitor activity; lipid binding; viral receptor activity

Biological Process: regulation of complement activation, classical pathway