Human Integrin Alpha 5 (ITGa5) RTU ELISA Kit | ITGa5 rtu elisa kit

Human Integrin Alpha 5 (ITGa5) ELISA Kit

Cat. Num. AAA4500517

• Gene Names: ITGA5; FNRA; CD49e; VLA-5; VLA5A
• Reactivity: Human
• Synonyms: Integrin Alpha 5 (ITGa5); Human Integrin Alpha 5 (ITGa5) ELISA Kit; CD49e; CD49-E; ITG-A5; FNRA; VLA5A; Fibronectin Receptor; Alpha Polypeptide; Integrin alpha-F; CD49 antigen-like family member E; ITGa5 rtu elisa kit

• Reactivity: Human
• Sequence Length: 1049

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGa5 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ITGa5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ITGa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITGa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ITGa5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1017029567
• NCBI GeneID: 3678
• NCBI Accession #: NP_002196.4
• NCBI GenBank Nucleotide #: NM_002205.4
• UniProt Accession #: P08648; Q96HA5
• Molecular Weight: 114,536 Da
• NCBI Official Full Name: integrin alpha-5
• NCBI Official Synonym Full Names: integrin subunit alpha 5
• NCBI Official Symbol: ITGA5
• NCBI Official Synonym Symbols: FNRA; CD49e; VLA-5; VLA5A
• NCBI Protein Information: integrin alpha-5
• UniProt Protein Name: Integrin alpha-5
• Protein Family: Integrin
• UniProt Gene Name: ITGA5
• UniProt Synonym Gene Names: FNRA

NCBI Description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]

Uniprot Description

Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430).